KRAS基因第12、13号密码子突变与肝内胆管癌临床病理的关系

发布时间：2018-04-02 15:13

本文选题：肝内胆管癌　切入点：KRAS基因　出处：《湖南师范大学》2016年硕士论文

【摘要】：【目的】探讨KRAS基因第12、13号密码子突变与肝内胆管癌临床病理的关系。【方法】收集近一年内的肝内胆管癌的石蜡包埋组织标本40例。常规脱蜡后,提取肝内胆管癌石蜡包埋组织标本中的DNA,用PCR技术对提取的DNA中的KRAS基因片段进行扩增和测序。以肝内胆管癌中KRAS基因2号外显子上第12、13密码子的突变作为检测对象。统计KRAS基因2号外显子上第12、13号密码子的突变表达谱,计算肝内胆管癌中KRAS基因的突变率,对比发生KRAS基因突变的肝内胆管癌患者与无KRAS基因突变的肝内胆管癌患者之间的临床病理资料,分析KRAS基因第12、13号密码子的突变与肝内胆管癌临床病理的关系。【结果】40例肝内胆管癌石蜡包埋组织标本中,发生KRAS基因突变13例(32.5%),其中12号密码子发生突变12例(92.3%),13号密码子发生突变1例(7.7%)。GGTGAT位点突变8例(61.5%),GGTGTT位点突变3例(23.1%),GGTGCT突变1例(7.7%),GGCGAC位点突变1例(7.7%)。KRAS基因第12、13号密码子的突变与患者的性别、年龄、肿瘤大小、肿瘤分型、肿瘤病理类型、分化程度、TNM分期、是否合并肝胆管结石、术前血清中CA199、CEA是否升高,各组间比较,差异无统计学意义(c2,P0.05);与肝内胆管癌的淋巴转移及远处转移的发生率存在相关性(c2,P0.05)。【结论】肝内胆管癌中KRAS基因的突变主要发生在12号密码子上,最常见突变位点为GGTGAT、GGTGTT突变。肝内胆管癌中KRAS基因第12、13号密码子的突变可能与肝内胆管癌的淋巴转移及远处转移存在关联,发生KRAS基因突变的肝内胆管癌具有更高的淋巴转移及远处转移发生率,临床检测KRAS基因第12、13号密码子的突变可能是评估肝内胆管癌预后的可靠指标。
[Abstract]:[objective] to investigate the relationship between the mutation of codon 12,13 of KRAS gene and clinicopathology of intrahepatic cholangiocarcinoma. [methods] 40 cases of paraffin embedded tissues of intrahepatic cholangiocarcinoma were collected within one year.After conventional dewaxing, DNA was extracted from paraffin embedded tissues of intrahepatic cholangiocarcinoma, and the KRAS gene fragment of DNA was amplified and sequenced by PCR technique.The mutation of codon 12 on exon 2 of KRAS gene was detected in intrahepatic cholangiocarcinoma.The mutation expression profiles of codon 12,13 on exon 2 of KRAS gene were analyzed, and the mutation rate of KRAS gene in intrahepatic cholangiocarcinoma was calculated.The clinicopathological data of intrahepatic cholangiocarcinoma patients with KRAS gene mutation and those without KRAS gene mutation were compared.To analyze the relationship between mutation of codon 12,13 of KRAS gene and clinicopathology of intrahepatic cholangiocarcinoma. [results] in 40 paraffin-embedded tissues of intrahepatic cholangiocarcinoma,Mutation and patient's sex,Age, tumor size, tumor type, tumor pathological type, degree of differentiation, TNM stage, whether or not hepatolithiasis, whether serum CA199 CEA was elevated before operation, and the comparison among the groups.The mutation of codon 12,13 of KRAS gene in intrahepatic cholangiocarcinoma may be associated with lymphatic metastasis and distant metastasis of intrahepatic cholangiocarcinoma. The incidence of lymphatic metastasis and distant metastasis in intrahepatic cholangiocarcinoma with KRAS gene mutation is higher.Clinical detection of mutations in codon 12,13 of KRAS gene may be a reliable predictor of prognosis of intrahepatic cholangiocarcinoma.
【学位授予单位】：湖南师范大学
【学位级别】：硕士
【学位授予年份】：2016
【分类号】：R735.8

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