金属胁迫下灵芝寡肽转运蛋白基因家族的转录表达

发布时间：2018-04-02 21:38

  本文选题：生长量　切入点：生物量　出处：《微生物学通报》2017年09期

【摘要】：【目的】寡肽转运蛋白(Oligopeptide transporters,OPTs)通过细胞质膜,将细胞外环境中的物质转入细胞内,从而参与各种生理活动。植物中OPTs蛋白参与金属运输和分配,从而促进植物对金属的利用和适应,但真菌中OPTs是否参与这一过程的相关报道较少。探究OPTs是否在灵芝对金属的适应过程中发挥作用。【方法】以灵芝荣保1号为材料,利用平板实验分析不同浓度的Cu2+、Fe2+和Se4+对菌丝体生长和生物量的影响,并采用实时荧光定量PCR分析不同金属离子胁迫下,两个培养阶段(15 d和30 d)灵芝寡肽转运蛋白基因(OPTs)的转录表达水平。【结果】Cu2+、Fe2+和Se4+三个金属离子在实验使用的浓度范围内抑制灵芝菌丝体的生长量和生物量,且在相同处理条件下的生长量与生物量的变化趋势相同。荧光定量PCR结果显示,除未检测到转录本的3个基因(OPT7、OPT8和OPT9),其余10个基因均表现出了差异性表达模式。在培养前期(15 d),有6个寡肽转运蛋白基因(OPT1、OPT3-6和OPT10)的相对表达量在Cu2+的胁迫下发生上调,而在Fe2+和Se4+的胁迫下,所有的OPTs基因的表达量均被下调。在培养后期(30 d),Se4+胁迫下的OPTs基因的表达量仍旧被下调,而Cu2+和Fe2+的胁迫下表达量均显著上调。【结论】金属能够在不同的程度上影响灵芝的生长量和生物量,同时寡肽转运蛋白基因对这些金属胁迫在转录水平上做出了响应,表明寡肽转运蛋白基因可能在灵芝对金属的适应过程中发挥作用。
[Abstract]:[objective] Oligopeptide transporters OPTs (Oligopeptide transportersOPTs) were transfered into the cells through the cytoplasmic membrane and involved in various physiological activities.OPTs proteins in plants are involved in metal transport and distribution, thus promoting the utilization and adaptation of plants to metals. However, there are few reports on whether OPTs is involved in this process in fungi.To explore whether OPTs plays a role in the adaptation of Ganoderma lucidum to metal. [methods] the effects of Cu2 Fe2 and Se4 on mycelium growth and biomass were studied by using Ganoderma lucidum Rong Bao 1 as a material.The real-time fluorescence quantitative PCR was used to analyze the stress of metal ions.Transcriptional expression level of Ganoderma lucidum oligoseptide transporter gene (OPTs) was observed at 15 d and 30 d after incubation. [results] Cu2 Fe2 and Se4 inhibited the growth and biomass of Ganoderma lucidum mycelium in the concentration range used in the experiment.And under the same treatment conditions, the change trend of biomass and growth is the same.Fluorescence quantitative PCR showed that all the other 10 genes showed different expression patterns except for 3 genes (OPT7OPT8 and OPT9G) which were not detected in transcripts.The relative expression of OPT1OPT3-6 and OPT10) was up-regulated under the stress of Cu2, but the expression of all OPTs genes was down-regulated under the stress of Fe2 and Se4.The expression of OPTs gene was still down-regulated at 30 days after culture, while that of Cu2 and Fe2 increased significantly. [conclusion] Metal can affect the growth and biomass of Ganoderma lucidum in different degree.At the same time, the oligoseptide transporter genes are responsive to these metal stresses at the transcriptional level, suggesting that the oligopeptide transporter genes may play a role in the adaptation of Ganoderma lucidum to metals.
【作者单位】： 四川农业大学资源学院微生物系;
【基金】：四川省教育厅重点项目(No.15ZA0013)~~
【分类号】：S567.31;X173
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本文编号：1702211