rna silencing 在 农作物 分类中 的翻译结果
本文关键词:Waxy基因的RNA沉默使转基因小麦种子中直链淀粉含量下降(英文),由笔耕文化传播整理发布。
RNA Silencing and Its Application in Plant Gene Function Research and Crop Improvement
RNA沉默及其在植物基因功能研究和作物改良中的应用
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RNA Silencing of Waxy Gene Results in Low Levels of Amylose in the Seeds of Transgenic Wheat (Triticum aestivum L.)
Waxy基因的RNA沉默使转基因小麦种子中直链淀粉含量下降(英文)
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Comparison of RNA Silencing Inducing Efficiency of Three PVX CP Gene Constructs via Transient Expression
瞬时表达比较马铃薯X病毒CP基因3种结构对RNA沉默的诱导效果
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The influence of temperature on RNA silencing and the level of resistance in transgenic tobacco plants was studied by using Northern blotting and ELISA to detect the virus in T3 plants that contain untranslatable PVYN CP gene.
本研究以转不可翻译的马铃薯Y病毒坏死株系外壳蛋白基因(PVYN CP)烟草的T3代植株为材料,在获得高度抗病植株并证明转基因植株的抗病性是由RNA沉默介导的基础上,采用Northern杂交及ELISA检测病毒的方法,分析了温度对转基因烟草中RNA沉默以及转基因植株抗病水平的影响。
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In this study, in order to effectively transcribe dsRNA in plants, an inducer of RNA silencing, two copies of Tobacco mosaic virus (TMV) partial movement protein gene (△MP) were ligated with soybean intron in inverted repeat manner, the recombinant was then inserted into binary vector pBIN438 under control of the 35S promoter.
本研究以在植物体内转录出RNA沉默的高效诱导因子—双链RNA(dsRNA)为目标,,将烟草花叶病毒(TMV)部分移动蛋白基因(ΔMP)以反向重复的方式与大豆内含子相连,并定向插入到植物表达载体pBIN438上35S启动子下游;
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Semi-quantitative RT-PCR assay demonstrated that NbTOM1 mRNA accumulation was inhibited in N. benthamiana. These results suggest that inhibition of NbTOM1 via RNA silencing is a potentially useful method for generating TMV-resistant plants and virus induced gene silencing is is a useful method for identification of gene function.
本论文证明通过沉默NbTOM1是一种有效的获得抗TMV植株的方法,同时表明病毒诱导的基因沉默是一种切实有效的鉴定基因功能的工具。
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These results could be used for further research of Rubisco subunit gene function and mechanism of RNA silencing.
本实验为进一步研究Rubisco小亚基基因功能和基因沉默机制奠定了基础。
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Plant expression vectors with untranslatable transgene arranged as sense, antisense orientation and inverted repeat were constructed with the full-length potato virus X (PVX) coat protein (CP) gene and their efficiency of RNA silencing inducing was analyzed after transient expression via agroinfiltration.
用全长马铃薯X病毒(PVX)CP基因构建非翻译的正义、反义和反向重复结构转基因的植物表达载体,通过农杆菌渗入法瞬时表达测试了这些转基因对RNA介导抗性的诱导效率和有效性.
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本文关键词:Waxy基因的RNA沉默使转基因小麦种子中直链淀粉含量下降(英文),由笔耕文化传播整理发布。
本文编号:170912
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