北京油鸡和来航鸡脾脏差异表达基因的筛选与分析

发布时间：2018-04-04 22:19

本文选题：脾脏　切入点：差异表达　出处：《中国兽医学报》2017年05期

【摘要】：为筛选北京油鸡和来航鸡脾脏组织中与抗病力差异相关的候选基因和信号通路,本研究采用高通量测序技术,构建了42日龄北京油鸡(抗病力较强)和来航鸡(抗病力相对较弱)脾脏组织的数字基因表达谱,对差异表达基因进行了GO功能分类、KEGG信号通路分析和STRING互作网络构建,并利用荧光定量PCR验证了部分差异表达基因。结果表明,以来航鸡为对照组,北京油鸡与之相比,差异倍数在2倍以上的共有1335个基因,其中693个上调表达,643个下调表达,包括多个与淋巴细胞的激活、分化和增殖、免疫器官发育等相关的基因,主要涉及到结合功能、细胞组成、细胞加工和生物学调节等功能。对通路显著性分析发现,与免疫相关生物学通路共有4个,其中BCR信号和TLR信号涉及脾脏B细胞介导的体液免疫应答反应,两条通路上的差异表达基因构成1个相互连接的互作网络,其中D79B、CD44、IL1B、SOCS1及TLR4等位于重要节点位置。为今后挖掘新基因和研究影响鸡抗病力的遗传因素等提供理论依据。
[Abstract]:In order to screen candidate genes and signaling pathways in spleen tissues of Beijing and Leheng chickens, high throughput sequencing technique was used in this study.The digital gene expression profiles of spleen tissues of 42-day-old Beijing fowl (with strong disease resistance) and Leheng chicken (relatively weak) were constructed, and the differential expression genes were analyzed by go functional classification and constructed by STRING interaction network.Some differentially expressed genes were verified by fluorescence quantitative PCR.The results showed that there were 1335 genes in Beijing fowl compared with the control group, 693 of them were up-regulated and 643 were down-regulated, including several genes which were related to lymphocyte activation, differentiation and proliferation.The development of immune organs and other related genes, mainly related to binding function, cell composition, cell processing and biological regulation and other functions.Significant analysis of the pathway showed that there were 4 immune-related biological pathways, in which BCR signal and TLR signal were involved in humoral immune response mediated by splenic B cells.The differentially expressed genes in the two pathways form an interlinked interaction network, in which D79BU CD44-IL1BNSOCS1 and TLR4 are located at important nodes.It provides theoretical basis for digging new genes and studying genetic factors affecting chicken disease resistance in the future.
【作者单位】：河北农业大学动物科技学院;河北科技师范学院;
【基金】：国家青年科学基金资助项目(31001003) 河北省教育厅优秀青年科学基金资助项目(Y2011118) 河北省自然科学基金资助项目(C2012407009) 河北省高等学校创新团队领军人才培育计划资助项目(LJRC004) 河北省现代农业攻关计划资助项目(15226302D)
【分类号】：S831

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