巴马香猪ATP合成酶基因表达发育变化与肉质性状相关性研究

发布时间：2018-04-05 16:28

本文选题：巴马香猪　切入点：ATP合成酶　出处：《黑龙江畜牧兽医》2017年15期

【摘要】：为了探讨ATP5B、ATP5H基因在巴马香猪和长白猪不同生长阶段背最长肌中的表达丰度与肉质性状之间的关系,揭示ATP合成酶基因对肉质的影响,试验采用荧光定量PCR技术分析了不同时期背最长肌中ATP5B、ATP5H基因mRNA表达量。结果表明:ATP5B、ATP5H基因mRNA表达量具有显著的年龄依赖变化,随着年龄的增加而呈下降趋势,在0日龄时明显高于其他日龄。0日龄时巴马香猪肌肉组织中ATP5B基因mRNA表达量显著高于长白猪(P0.05),ATP5H基因mRNA表达量显著低于长白猪(P0.05)。巴马香猪肌肉组织中ATP5B基因mRNA表达量与电导率、剪切力呈显著的负相关性(P0.05),ATP5H基因mRNA表达量与剪切力呈极显著的负相关性(P0.01),与肌内脂肪含量呈显著的负相关性(P0.05)。长白猪肌肉组织中ATP5B、ATP5H基因mRNA表达量与肉色、电导率、剪切力、肌内脂肪含量均无显著的相关性(P0.05)。
[Abstract]:In order to investigate the relationship between the expression abundance of ATP-5B gene in longissimus dorsi muscle of Bama pig and Landrace pig at different growth stages, and to reveal the effect of ATP synthase gene on meat quality, the relationship between the expression abundance and meat quality of longissimus dorsi muscle in different growth stages was studied.The results showed that the mRNA expression of the mRNA gene of the 5 ~ (- 5) ATP _ (5B) gene had a significant age-dependent change, and decreased with the increase of age.The mRNA expression of ATP5B gene in muscle tissue of Bama Xiang pig was significantly higher than that of P0.05 P0.05H gene of Landrace pig at 0 day old and significantly lower than that of Landrace pig P0.05H gene at 0 day old.There was a significant negative correlation between the expression of ATP5B gene mRNA and electrical conductivity and shear stress in the muscle tissue of Bama Xiang pig. The expression of P0.05H gene mRNA was significantly negatively correlated with the shear stress, and there was a significant negative correlation between the expression of mRNA and the content of intramuscular fat.There was no significant correlation between mRNA expression and meat color, electrical conductivity, shear stress and intramuscular fat content in muscle tissue of Landrace pigs.
【作者单位】：广西大学动物科学技术学院;广西壮族自治区畜牧研究所;
【基金】：国家自然科学基金项目(81360135) 广西家畜遗传改良重点实验室自主研究课题项目(2015GXKLLGI-06)
【分类号】：S828

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