小麦BNS雄性不育中国春恢复基因的连锁群检测和QTL初步定位

发布时间：2018-04-08 16:52

本文选题：小麦　切入点：BNS雄性不育　出处：《麦类作物学报》2016年07期

【摘要】：BNS是一个新发现的温敏小麦雄性不育系,有良好的不育性和自身转换性,在杂交小麦利用和不育资源研究中有重要价值。为定位BNS的恢复基因,首先以BNS的高恢复系中国春为材料,创建BNS×中国春F2作图群体,建立自交结实率和花粉可育率两个表型BSA池;然后用中国春缺体-四体系检测恢复相关连锁群;最后用这些连锁群上的SSR分子标记筛选BSA池,用检测的连锁标记筛选F2作图群体,进一步定位恢复基因的QTL位点。结果表明,用BNS与中国春缺四体杂交,根据F1不育性检测到4个相关连锁群,分别是1A、1B、2B和7B;利用4个相关连锁群和4个非相关连锁群共8个染色体上的222对SSR分子标记筛选2对共4个BSA池,结果在3个相关连锁群上检测到8对连锁标记;用这8对连锁标记筛选F2群体210个个体植株,检测到5个QTL位点,位于1A、1B和2B染色体上。这些位点中,1个与自交结实率相关,2个与两个表型均相关,是主效QTL位点,另2个与花粉可育率相关,是微效QTL位点。这些结果为BNS恢复基因分子标记选择和精细定位奠定了基础。
[Abstract]:BNS is a newly discovered thermo-sensitive wheat male sterile line with good sterility and self-transformation. It is of great value in the utilization of hybrid wheat and the study of sterile resources.In order to locate the restorer gene of BNS, the high restorer line of BNS, Chinese Spring, was used as the material to create the BNS 脳 Chinese spring F2 mapping population, and two phenotypic BSA pools of self-fertility rate and pollen fertility rate were established.At last, the SSR molecular markers on these linkage groups were used to screen the BSA pool, the F2 mapping population was screened by the detected linkage markers, and the QTL loci of the restorer gene were further located.The results showed that four linkage groups were detected according to the sterility of F _ 1 by BNS crossing with Chinese spring deficient tetralogy.Two pairs of BSA pools were screened by 222 pairs of SSR markers on 8 chromosomes of 4 and 4 unrelated linkage groups, respectively, and 8 pairs of linkage markers were detected in 3 linkage groups.The 8 pairs of linkage markers were used to screen 210 individual plants of F2 population and 5 QTL loci were detected on 1A1B and 2B chromosomes.Of these loci, one was related to self-fertilization rate, two to two phenotypes, which were major QTL loci, and the other two to pollen fertility, which were microfunctional QTL loci.These results provide a basis for molecular marker selection and fine mapping of BNS restoration genes.
【作者单位】：河南科技学院/河南省高等学校作物分子育种重点学科开放实验室/现代生物育种河南省协同创新中心;四川农业大学农学院;
【基金】：河南省基础与前沿计划重点项目(122300410011,162300410136)
【分类号】：S512.1

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