大白菜根肿病的遗传规律及抗病基因定位研究

发布时间：2018-04-08 23:00

本文选题：大白菜　切入点：根肿病　出处：《华北农学报》2017年04期

【摘要】：为探究根肿病抗病遗传规律,采用根肿病抗性差异的材料G57和G70,构建了包含500个单株的F2分离群体。通过对父母本、F1及F2分离群体的人工接种表型鉴定结果进行统计分析,结果发现,材料中的根肿病抗性由显性单基因控制。为进一步定位试验材料中的抗病基因,利用678个分子标记对双亲、F1及F2群体进行了筛选验证,初步将抗病基因定位在分子标记KBRH129J18和TCR02-F。并基于2个连锁标记,开发设计了更紧密连锁的分子标记,最终获得与抗病基因连锁的5对SSR分子标记,分别为Bra0345-1、Bra0235-2、Bra0235-1、Bra19317、Bra019392。其与抗病基因的遗传距离依次为2.4,2.4,2.4,2.5,3.3 c M。开发设计的多态性标记经验证在大白菜中具有较好的适用性。
[Abstract]:In order to study the genetic law of resistance to rhizomegaly disease, F _ 2 segregation population containing 500 plants was constructed by using G57 and G70, which were different in resistance to root swelling.The results of phenotypic identification of parents of F _ 1 and F _ 2 were statistically analyzed. The results showed that the resistance to root swelling was controlled by dominant single gene.In order to further localize the resistance genes in the experimental materials, 678 molecular markers were used to screen and verify the parents F _ 1 and F _ 2, and the resistance genes were preliminarily located in the molecular markers KBRH129J18 and TCR 02-F.Based on two linkage markers, a more closely linked molecular marker was developed and designed. Finally, five pairs of SSR markers linked to disease resistance gene were obtained, which were Bra0345-1nB0235-2Cor Bra19317B019392. the molecular markers were identified as Bra0345-1, Bra0235-2, Bra0235-1, Bra0235-1, Bra0235-1, Bra0235-1.The genetic distance between the gene and the resistant gene was 2. 4 and 2. 4%, 2. 4 and 2. 5. 3. 3 c M.The polymorphic markers developed and designed were proved to be suitable for Chinese cabbage.
【作者单位】：天津科润蔬菜研究所蔬菜种质创新国家重点实验室天津市蔬菜遗传育种企业重点实验室;
【基金】：国家重点研发计划项目(2016YFD0101701) 国家现代农业产业技术体系项目(CARS-25-G-02) 天津市自然科学基金项目(16JCYBJC29300) 天津市科技创新体系及平台建设计划项目(16PTSYJC00260) 农业部“农业科研杰出人才培养计划”项目
【分类号】：S436.341

【相似文献】