MC1R与Slc7a11基因多态性与哈萨克羊毛色相关的研究

发布时间：2018-04-09 04:11

本文选题：哈萨克绵羊　切入点：MCR基因　出处：《中国畜牧杂志》2017年10期

【摘要】：本研究采集新疆伊犁哈萨克自治州特克斯县4种不同被毛颜色2~3周岁的220只哈萨克母羊血样用于提取DNA,其中黑色被毛羊52只,棕色被毛羊52只,青灰色被毛羊59只,白色被毛羊57只;并采集绵羊左侧肩胛皮肤样本,每种毛色各10个,用于组织形态学观察。本研究运用PCR-SSCP技术及测序技术对MC1R基因及Slc7a11基因多态性及其对哈萨克羊毛色的影响进行研究。运用皮肤样本制作切片用于不同毛色组织形态学观察。结果表明:MC1R基因突变位点在白色和棕色被毛群体中,只存在BB基因型;而在黑色和青灰色被毛群体中,存在AA、AB和BB 3种基因型;且AB基因型均为优势基因型,A等位基因为优势等位基因。由组织形态学观察可知,黑色和青灰色被毛组织毛囊分布极为相似,毛囊分布稀疏且皮脂腺发达;棕色被毛最具特色,毛囊丛分布明显,皮脂腺分布不发达;白色被毛毛囊分布最为密集,且皮脂腺分布密集并且最为发达。经χ~2检验,MC1R基因的基因型频率在4种毛色羊中的分布差异极显著(P0.01)。Slc7a11基因位点在伊犁哈萨克羊群体中发现其不存在多态性。
[Abstract]:In this study, the blood samples of 220 Kazak sheep of 2 or 3 years of age were collected from Tekes County, Yili Kazak Autonomous Prefecture, Xinjiang. Among them, 52 were black wool sheep, 52 brown wool sheep and 59 blue grey wool sheep.The skin samples of left shoulder blades of 57 white wool sheep were collected, 10 of each color were used for histomorphological observation.In this study, PCR-SSCP and sequencing techniques were used to study the polymorphism of MC1R gene and Slc7a11 gene and their effects on the color of Kazak wool.Skin samples were used to make sections for histomorphological observation of different coat colors.The results showed that there were only BB genotypes in white and brown hairy populations, but only three genotypes in black and green grey hairy populations.The AB genotype was the dominant genotype and the allele was the dominant allele.According to histomorphology, the hair follicles of black and gray hair tissues are very similar, the hair follicles are sparse and the sebaceous glands are developed, the brown hair coat is the most characteristic, the hair follicle plexus is obviously distributed, and the sebaceous gland is not developed.White hair follicles are most densely distributed and sebaceous glands are densely distributed and most developed.The genotype frequency of MC1R gene was significantly different among the four kinds of wool sheep by 蠂 ~ 2 test. It was found that there was no polymorphism in Yili Kazak sheep population at the locus of P0.01U. Slc7a11.
【作者单位】：新疆农业大学动物科学学院;特克斯县畜牧兽医局;
【基金】：农业部体系项目(CARS-40) 疆农业大学产学研联合培养研究生示范基地项目(xjaucxy-yjs-20152011)
【分类号】：Q78;S826.84

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