TNFRSF1A基因CpG岛甲基化与基因表达及T淋巴细胞亚群指标的相关性分析

发布时间：2018-04-09 13:29

本文选题：TNFRSFA　切入点：甲基化　出处：《畜牧兽医学报》2017年10期

【摘要】：旨在探讨TNFRSF1A基因CpG岛的甲基化水平,阐明该基因甲基化与基因表达及T淋巴细胞亚群性状的关系。本研究以抗病力强的华北型黑猪大蒲莲猪和抗病力相对较弱的引进猪种长白猪的仔猪为试验群体,通过亚硫酸氢盐处理后测序法(BSP)探求TNFRSF1A基因上游CpG岛甲基化情况,并将甲基化位点的甲基化频率与基因表达量及T淋巴细胞亚群性状进行相关性分析。结果表明:1)TNFRSF1A基因起始位点附近预测到2个CpG岛(Island),跨这2个CpG岛进行BSP测序后共发现25个CpG位点,其中Island 1和Island 2中各有9个CpG位点,其余位于2个CpG岛之间。Island 1的甲基化频率在大蒲莲和长白猪两群体间差异不显著(P0.05),Island 2的甲基化频率大蒲莲猪群体显著高于长白猪群体(P0.05)。2)Island 2中甲基化频率与ΔCt值成正相关,即甲基化频率与基因表达量成负相关,其中+126、+128、+159、+162和+164是重要的甲基化位点。3)两个猪群体中位于Island 2的+159和+164位点与CD4+CD8+CD3+指标显著正相关(P0.05)。Island 2中+159和+164位点甲基化频率与基因表达量成负相关,而且这2个位点又与T淋巴细胞亚群CD4+CD8+CD3+显著正相关,笔者推测,大蒲莲仔猪TNFRSF1A基因+159和+164位点的甲基化频率增加,可能导致TNFRSF1A基因表达量下降,影响T淋巴细胞亚群性状,从而影响机体的免疫水平。
[Abstract]:The aim of this study was to investigate the methylation level of TNFRSF1A gene CpG island and to elucidate the relationship between methylation and gene expression and T lymphocyte subsets.In this study, we studied the methylation of CpG islands in the upper reaches of TNFRSF1A gene by using high-resistant North China black pig Pu Lin and piglets with relatively weak resistance to disease of imported Landrace pigs.The correlation between methylation frequency and gene expression and T lymphocyte subsets was analyzed.The results showed that two CpG islands were predicted near the initiation site of TNFRSF1A gene, and 25 CpG loci were identified by BSP sequencing across the two CpG islands, including 9 CpG loci in Island 1 and Island 2, respectively.There was no significant difference in methylation frequency between Dapulian and Landrace pigs in the other two CpG islands. The methylation frequency of Dapu Island 2 was significantly higher than that of Landrace Pig population (P 0.05). The methylation frequency was positively correlated with 螖 Ct value in Landrace White Pig population (P0.05) and Landrace Island 2, respectively.That is, the frequency of methylation is negatively correlated with the amount of gene expression.Among them, 126, 128, 159, 162 and 164 were important methylation sites. 3) there was a negative correlation between the methylation frequencies of 159 and 164 loci in Island 2 and the CD4 CD8 CD3 index.Moreover, these two loci were positively correlated with CD4 CD8 CD3 of T lymphocyte subsets. We speculate that the increase of methylation frequency of TNFRSF1A gene 159 and 164 in Pu Lin piglets may lead to the decrease of TNFRSF1A gene expression.The effect of T lymphocyte subsets, thus affecting the immune level of the body.
【作者单位】：山东省农业科学院畜牧兽医研究所/山东省畜禽疫病防治与繁育重点实验室;
【基金】：转基因生物新品种培育重大科技专项(2014ZX08009-045B) 山东省现代农业产业技术体系生猪创新团队建设项目(SDAIT-08-03) 山东省农业科学院农业科技创新工程“畜禽种质资源保护与共享利用”(CXGC2016A04) 山东省中青年科学家科研奖励基金(ZR2016CB15)
【分类号】：S828.2

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