大鼠正畸牙移动中NF-κB通路相关差异基因的GO分析

发布时间：2018-04-09 16:34

本文选题：正畸牙移动　切入点：核转录因子κB　出处：《口腔医学研究》2017年08期

【摘要】：目的:利用基因表达谱芯片对正畸加力组与正常大鼠牙齿牙周组织的基因表达谱进行对比分析,筛选差异基因并进行GO分析。方法:将6只SD大鼠随机分为加力组和对照组,加力组建立大鼠正畸牙齿移动模型。根据预实验结果,分别在0h、12h处死大鼠,并截取上颌第一磨牙及外周约1mm的牙周组织,提取总RNA,进行全基因组表达谱芯片检测,对差异表达基因进行GO分析,并运用RT-qPCR对芯片结果进行验证。结果:芯片结果显示,共筛选到463条差异基因,其中实验组与对照组相比250个基因表达上调,213个基因表达下调,在差异基因中涉及到NF-κB信号通路的基因有8个,均表达上调。GO分析涉及到1189个不同的功能分类。结论:正畸加力12h后牙齿牙周组织中与炎症反应相关的基因和与中性粒细胞趋化相关的基因表达上调。
[Abstract]:Aim: to compare and analyze the gene expression profiles in periodontal tissues of orthodontic plus force group and normal rats by using gene expression microarray to screen differentially expressed genes and go analysis.Methods: six Sprague-Dawley rats were randomly divided into treatment group and control group.According to the experimental results, the rats were killed at 0 h and 12 h, the periodontal tissues of maxillary first molar and peripheral 1mm were cut off, the total RNAs were extracted, the whole genome expression microarray was detected, and the differentially expressed genes were analyzed by go.The chip results are verified by RT-qPCR.Results: a total of 463 differentially expressed genes were screened, among which 250 genes were up-regulated and 213 genes were down-regulated in the experimental group compared with the control group. There were 8 genes involved in NF- 魏 B signaling pathway in the differentially expressed genes.All up-regulated. Go analysis involved 1189 different functional classifications.Conclusion: the expression of genes related to inflammation and neutrophil chemotaxis in periodontal tissues is up-regulated 12 h after orthodontic stress.
【作者单位】：南开大学医学院;天津市口腔医院正畸科;
【基金】：国家临床重点专科建设项目(口腔正畸专业)批准文号:国卫医办函[2013]544号天津市卫生计生委科技基金重点攻关项目(编号:14KG132)
【分类号】：R783.5

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