人外周血细胞放射损伤相关基因的生物信息学

发布时间：2018-04-10 11:20

本文选题：放射损伤 + 基因芯片　；参考：《解剖学报》2017年01期

【摘要】：目的从基因水平揭示放射前后人外周血细胞的变化。方法从基因表达数据库(GEO)中下载两组经放射处理后人外周血基因芯片数据,利用Qlucore Omics Explorer 3.0软件筛选差异表达基因,STRING、DAVID等在线分析工具对差异表达基因进行下一步的生物信息学分析。结果共筛选出94个共同差异表达基因,其中共同表达上调31个,共同表达下调11个,这些差异表达基因主要涉及到细胞凋亡的调控,细胞程序性死亡的调控,细胞死亡的调控,细胞周期的调控,DNA损伤应答,胞内信号转导等的分子功能及生物学过程。通过STRING分析,发现泛素C(UBC)、增殖细胞核抗原(PCNA)、鼠双微体基因-2(MDM2)处在核心节点位置,并参与p53通路。结论通过生物信息学的方法分析得出UBC、PCNA、MDM2可能成为潜在的防护放射后损伤的靶点。
[Abstract]:Objective to reveal the changes of human peripheral blood cells before and after radiation at the gene level.Methods two sets of human peripheral blood gene microarray data were downloaded from gene expression database,The next step of bioinformatics analysis of differentially expressed genes was carried out by using Qlucore Omics Explorer 3.0 software to screen differentially expressed genes and other online analysis tools, such as STRINGGY-DAVID.Results A total of 94 common differentially expressed genes were screened, among which 31 were up-regulated and 11 were down-regulated. These differentially expressed genes are mainly involved in the regulation of apoptosis, programmed cell death and cell death.Cell cycle regulation of DNA damage response, intracellular signal transduction and molecular functions and biological processes.By STRING analysis, it was found that ubiquitin (UBC), proliferating cell nuclear antigen (PCNA) and mouse double microbody gene (-2mMDM2) were located at the core node and involved in p53 pathway.Conclusion through bioinformatics analysis, it is concluded that UBCP-PCNAMA-MDM2 may be a potential target for protection against post-radiation injury.
【作者单位】：南方医科大学基础医学院基因工程研究所;
【基金】：国家青年自然科学基金(81502761)
【分类号】：R815

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