水稻斑点叶突变体spl101和spl102的筛选及候选基因鉴定

发布时间：2018-04-11 21:17

本文选题：水稻斑点叶突变体 + Mutmap　；参考：《遗传》2017年04期

【摘要】：斑点叶突变体是指植物在正常生长条件下叶片或叶鞘上自发形成、且与病原菌侵染产生的病斑类似的一类突变体,筛选并研究斑点叶突变体对揭示植物抗病反应机理具有重要意义。为了进一步研究斑点叶的形成机制,本文通过EMS诱变品种宽叶粳(KYJ),筛选得到两个斑点叶突变体spl101和spl102。这两个突变体在生长发育晚期(抽穗期以后)形成严重的类病斑。遗传分析表明,spl101和spl102均受隐性单基因控制。利用Mutmap方法对候选基因进行克隆,结果显示,spl101和spl102的候选基因均为OsEDR1,该基因与类病斑发生有关。在spl101中,OsEDR1基因突变发生在第6外显子和第6内含子的交接处,该突变导致第6内含子的错误识别,最终造成移码突变。在spl102中,OsEDR1基因突变发生在第10外显子上,导致一个苯丙氨酸(F)变成半胖氨酸(C)。因此,本研究鉴定了两个新的OsEDR1等位突变,对OsEDR1抗病反应机理的进一步研究及丰富水稻种质资源具有积极意义。同时验证了利用Mutmap方法克隆水稻突变基因的有效性。
[Abstract]:Spotted leaf mutants are a class of mutants that spontaneously form in the leaves or leaf sheaths of plants under normal growth conditions and are similar to the disease spots produced by pathogen infection.It is important to screen and study spot leaf mutants to reveal the mechanism of plant disease resistance response.In order to further study the mechanism of spot leaf formation, two spot leaf mutants spl101 and spl102 were screened by EMS mutagenesis.The two mutants developed severe disease-like spots at the late stages of growth and development (after heading date).Genetic analysis showed that both Spl101 and spl102 were controlled by recessive single gene.The candidate gene was cloned by Mutmap method. The results showed that the candidate genes of Spl101 and spl102 were OsEDR1, which was related to the occurrence of disease.The mutation of OsEDR1 gene occurred at the junction of exon 6 and intron 6 in spl101.In spl102, the mutation of OsEDR1 gene occurred at exon 10, resulting in the formation of a phenylalanine (phenylalanine) into a semipulgarine cationic acid.Therefore, two new OsEDR1 alleles were identified in this study, which have positive significance for the further study of the mechanism of OsEDR1 disease resistance and the enrichment of rice germplasm resources.At the same time, the Mutmap method was used to clone rice mutant gene.
【作者单位】：海南大学农学院海南省热带生物资源可持续利用重点实验室;中国科学院遗传与发育生物学研究所植物细胞与染色体工程国家重点实验室;中国科学院大学;
【基金】：植物细胞与染色体工程国家重点实验室自主研究课题~~
【分类号】：S511

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