盐角草PsaH基因的克隆及生物信息学分析

发布时间：2018-04-12 12:51

本文选题：盐角草 + SePsaH基因　；参考：《新疆农业科学》2017年09期

【摘要】：【目的】从盐生植物盐角草(Salicornia europaea)中克隆得到一个参与光合反应光系统I(PSI)复合蛋白H亚基的Psa H基因,分析该基因进行生物信息学,为研究该基因的作用奠定基础并为改良作物耐盐性分子育种提供候选基因。【方法】以盐生植物盐角草为实验材料,采用RT-PCR方法克隆Psa H基因,利用NCBI、MEGA、Expasy等生物信息学工具对其核酸序列、编码的氨基酸序列及蛋白质的结构和功能进行分析。【结果】克隆得到一个与耐盐有关的基因,命名为Se Psa H,属于光系统I(PSI)家族H亚基成员,其开放阅读框(ORF)为438 bp,基因编码145个氨基酸,预测蛋白分子量为15.3 k D,等电点9.84,是亲水性蛋白;系统进化树分析表明其与菠菜亲缘关系较近,通过对该蛋白保守性分析发现其含有4个保守性结构域。【结论】获得盐角草Se Psa H基因,为进一步研究该基因耐盐功能及其在盐生植物耐盐机制中的作用提供了基础。
[Abstract]:[objective] to clone a Psa H gene from Salicornia europaea, a complex protein H subunit involved in photosynthetic response light system, and analyze the gene for bioinformatics.In order to study the role of this gene and to provide candidate genes for improving crop salt-tolerant molecular breeding. [methods] Psa H gene was cloned by RT-PCR with halophytic plant halophytes as experimental material.The nucleic acid sequence, encoded amino acid sequence and the structure and function of protein were analyzed by bioinformatics tools such as NCBI MEGAGA-Expasy. [results] A salt-tolerant gene was cloned.Se Psa H, a member of H subunit of the light system Igna PSI family, is an open reading frame (ORF) of 438bp. the gene encodes 145 amino acids, the predicted protein molecular weight is 15.3kD, and the isoelectric point is 9.84. it is a hydrophilic protein.Phylogenetic tree analysis showed that it was closely related to spinach. The conserved protein contained four conserved domains. [conclusion] se Psa H gene was obtained.It provides a basis for further studying the salt tolerance function of the gene and its role in the mechanism of salt tolerance in halophytes.
【作者单位】：新疆农业科学院核技术生物技术研究所;
【基金】：新疆维吾尔自治区自然科学基金项目“碱蓬SsPSI基因提高转基因烟草耐旱性的研究”(2015211A028) 新疆农业科学院优秀青年科技人才基金“盐生植物盐角草耐盐基因的发掘与功能研究”(xjnky-2012-003)~~
【分类号】：Q943.2;S156.4

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