版纳微型猪近交系LRWD1基因克隆及功能生物信息学分析

发布时间：2018-04-12 23:33

本文选题：富含亮氨酸的重复序列(LRR) + 色氨酸-天冬氨酸重复序列(WD)　；参考：《云南农业大学学报(自然科学)》2017年01期

【摘要】：LRWD1基因在精子形成过程中起重要作用。从Gen Bank下载猪及近缘物种的LRWD1 mRNA序列作为参考序列,设计特异引物扩增版纳微型猪近交系(BMI)LRWD1基因。对蛋白质序列进行功能生物信息学分析,构建多物种系统进化树。研究获得了BMI LRWD1 1 944 bp的编码区序列(Gen Bank登录号:KU705639,对应的氨基酸登录号:AOC89057),编码647个氨基酸,蛋白质分子量(Mw)为71.40 k D,等电点(p I)为7.52。功能生物信息学分析表明LRWD1蛋白质存在2个保守结构域,无跨膜结构,无信号肽序列;N末端和C末端均疏水;有6类功能活性位点,位于细胞质的概率是70.6%。系统进化分析表明,BMI与狗、大熊猫的亲缘关系最近。研究结果将为进一步研究LRWD1基因在猪精子发生方面的作用及功能奠定基础。
[Abstract]:LRWD1 gene plays an important role in spermatogenesis.The LRWD1 mRNA sequence of porcine and related species was downloaded from Gen Bank as reference sequence, and specific primers were designed to amplify the LRWD1 gene of BMIN mini-pig inbred line BMIWD1.The functional bioinformatics analysis of protein sequences was carried out to construct the phylogenetic tree of multiple species.The coding region sequence of BMI LRWD1 1,944bp was obtained. The accession number of Gen Bank was: KU705639, the corresponding amino acid login number was: AOC89057, encoding 647 amino acids, the molecular weight of protein was 71.40kD, and the isoelectric point was 7.52.Functional bioinformatics analysis showed that LRWD1 protein had two conserved domains, no transmembrane structure, no signal peptide sequence, both N-terminal and C-terminal hydrophobic, and there were six functional active sites, the probability of being located in cytoplasm was 70.6.Phylogenetic analysis showed that BMI had the closest relationship with dogs and giant pandas.The results will lay a foundation for further study on the role and function of LRWD1 gene in porcine spermatogenesis.
【作者单位】：云南农业大学云南省版纳微型猪近交系重点实验室;云南农业大学动物科学技术学院;云南省家畜改良工作站;
【基金】：国家自然科学基金项目(31660637,31460580,31660650)
【分类号】：S828.2

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