ST酿酒酵母基因工程菌对大鼠肠道菌群的影响

发布时间：2018-04-13 00:00

本文选题：ST酿酒酵母基因工程菌 + 末端限制性片段长度多态性　；参考：《黑龙江畜牧兽医》2017年19期

【摘要】：为了研究ST酿酒酵母基因工程菌对大鼠肠道菌群的影响,试验将90只SPF级SD大鼠随机分为空白对照组、工程菌组及酵母菌组,分别灌服生理盐水、工程菌菌液、酿酒酵母菌菌液,持续21 d后连续3 d灌服大肠杆菌H10407菌液,分别收集灌胃7,14,21天及攻毒后的各组大鼠粪便,提取粪便细菌DNA,利用末端限制性片段长度多态性(T-RFLP)、实时荧光定量PCR检测了ST酿酒酵母基因工程菌对肠道菌群微生物可操作单元(OTU)和肠道5种重要细菌(类杆菌、梭菌、肠球菌、双歧杆菌和乳杆菌)数量的变化,并测定了肠道菌群pH值。结果表明:在灌胃期间,工程菌组及酵母菌组的OTU值和双歧杆菌、梭菌数量极显著高于空白对照组(P0.01),其他细菌数量均不同程度升高。各组pH值均呈现下降趋势,第21天时,工程菌组及酵母菌组pH值极显著低于空白对照组(P0.01)。攻毒产肠毒素大肠杆菌后,工程菌OTU值和类杆菌、乳杆菌、双歧杆菌数量均极显著高于其他两组(P0.01),pH值极显著高于空白对照组(P0.01)。与灌服前相比,工程菌组的OTU值、细菌数量及pH值均变化幅度最小。说明ST酿酒酵母基因工程菌能够改善肠道菌群结构,丰富肠道菌群多态性,降低pH值,增加有益菌群的数量。当动物机体受到产肠毒素大肠杆菌感染时,ST酿酒酵母基因工程菌能够有效降低产肠毒素大肠杆菌对肠道菌群的破坏,发挥预防保护作用,维持肠道内菌群稳定。
[Abstract]:In order to study the effect of genetically engineered strains of St Saccharomyces cerevisiae on intestinal flora of rats, 90 SD rats of SPF grade were randomly divided into three groups: blank control group, engineering bacteria group and yeast group.The liquid of Saccharomyces cerevisiae lasted 21 days and then was administered with Escherichia coli H10407 for 3 days. The feces of each group of rats were collected for 21 days after oral administration.Fecal bacterial DNA was extracted, and real-time fluorescent quantitative PCR was used to detect five important bacteria (Bacteroid bacillus, Clostridium spp, Enterococcus) and five important enteric bacteria (Bacteroid, Clostridium and Enterococcus), which were operated by ST-Saccharomyces cerevisiae.The number of bifidobacterium and Lactobacillus) and the pH value of intestinal flora were determined.The results showed that the OTU and Bifidobacterium counts of the engineering and yeast groups were significantly higher than those of the control group (P 0.01), and the other bacteria counts were increased in varying degrees during gastric perfusion.On the 21st day, the pH value of the engineering bacteria group and the yeast group was significantly lower than that of the blank control group (P 0.01).After enterotoxigenic Escherichia coli, the OTU value and the number of bacilli, Lactobacillus and Bifidobacterium were significantly higher than those of the other two groups.Compared with before administration, the OTU value, bacterial quantity and pH value of the engineering bacteria group were the smallest.The results showed that the genetically engineered strains of St Saccharomyces cerevisiae could improve the structure of intestinal flora, enrich the polymorphism of intestinal flora, reduce pH value and increase the number of beneficial flora.When the animal body is infected with enterotoxigenic Escherichia coli, the genetically engineered strain of S.cerevisiae St can effectively reduce the destruction of enterotoxigenic Escherichia coli to the intestinal flora, play a preventive and protective role, and maintain the stability of intestinal flora.
【作者单位】：吉林农业大学动物科学技术学院;军事医学科学院军事兽医研究所;吉林省人兽共患病预防与控制重点实验室;
【分类号】：S816

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