盐穗木水通道蛋白基因的克隆与功能鉴定

发布时间：2018-04-13 17:40

本文选题：盐穗木 + 盐胁迫　；参考：《基因组学与应用生物学》2017年11期

【摘要】：非生物胁迫(如盐渍和干旱)会引起植物水分代谢的紊乱,导致植物细胞水分丧失,直接抑制植物的生长发育。研究水通道蛋白(aquaporins,AQPs)在非生物胁迫下对植物生理功能的影响十分重要。本研究利用RACE技术克隆获得一个盐穗木(Halostachys caspica)水通道蛋白家族亚类质膜内嵌蛋白(plasma intrinsic protein,PIPs)基因,命名为Hc PIP1。Hc PIP1基因全长序列为1 244 bp,包含858 bp的开放阅读框(open reading frame,ORF)序列,编码285个氨基酸,分子量大小约为30.6 k D。q RT-PCR检测表明Hc PIP1在盐穗木根部的表达量显著高于同化枝中,盐胁迫诱导其上调表达。在酿酒酵母INVSc1中Hc PIP1的异源过表达显著提高了重组菌的耐盐能力;和野生型植株相比过表达Hc PIP1的拟南芥能够显著缓解渗透胁迫和离子胁迫对生长的抑制,并且根长明显比野生型的长。结果表明Hc PIP1在胁迫时能够通过增加根的生长以抵御胁迫的影响。
[Abstract]:Abiotic stress (such as salinization and drought) can lead to the disorder of water metabolism in plants, resulting in the loss of water in plant cells, which directly inhibits the growth and development of plants.It is very important to study the effects of aquaporins aquaporins on plant physiological function under abiotic stress.In this study, RACE technique was used to clone a aquaporin-like protein family of Halostachys caspica. the gene was named Hc PIP1.Hc PIP1 (1 244bp), which contained 858 BP open reading frame (ORF) sequence, and Halostachys caspicia (Halostachys caspicia) gene was cloned into Halostachys caspicia.The results showed that the expression of HC PIP1 in the roots of salted panicle was significantly higher than that in the assimilation branch, and the expression was up-regulated by salt stress.The heterologous expression of HC PIP1 in Saccharomyces cerevisiae INVSc1 significantly increased the salt tolerance of the recombinant strain, and compared with wild type plants, Arabidopsis thaliana, which overexpressed Hc PIP1, significantly alleviated the inhibition of osmotic stress and ion stress on the growth of Arabidopsis thaliana.And the root length is obviously longer than that of the wild type.The results showed that HC PIP1 could resist stress by increasing root growth.
【作者单位】：新疆大学生命科学与技术学院新疆生物资源基因工程重点实验室;
【基金】：新疆重点实验室专项资金项目(2014KL001)资助
【分类号】：Q943.2

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