模拟失重对大肠杆菌K12基因表达及表型的影响

发布时间：2018-04-14 12:01

本文选题：模拟失重 + 大肠杆菌　；参考：《微生物学通报》2017年05期

【摘要】：【目的】通过低剪切力模拟失重(Low-shear modeled microgravity,LSMMG)连续传代培养大肠杆菌,检测大肠杆菌在模拟失重条件下的表型变化及基因改变。【方法】利用旋转细胞培养系统模拟失重环境对大肠杆菌K12进行连续传代培养,对菌株进行增殖速率、耐酸性和生物膜形成的测定,以此评估LSMMG对大肠杆菌K12表型的影响。利用转录组测序检测模拟失重条件下差异表达的基因,与表型作比对。【结果】模拟失重导致大肠杆菌增殖速率降低,耐酸性下降,生物膜形成能力增强;模拟失重条件下,营养代谢相关差异表达基因有25个,其中20个表达下降,2个与耐酸相关基因表达均下降。【结论】模拟失重会引起大肠杆菌表型及相应的基因变化,其中生物膜形成能力的增强可能对航天飞行造成潜在威胁。
[Abstract]:[objective] to culture Escherichia coli by continuous passage of Low-shear modeled microgravimetric LSMMG by simulating weightlessness with low shear stress.The phenotypic changes and gene changes of Escherichia coli under simulated weightlessness were detected. [methods] Escherichia coli K12 was cultured continuously by rotating cell culture system under simulated weightlessness.Acid tolerance and biofilm formation were measured to evaluate the effect of LSMMG on the phenotype of Escherichia coli K12.Transcriptome sequencing was used to detect differentially expressed genes under simulated weightlessness, compared with phenotypes. [results] simulated weightlessness resulted in a decrease in E. coli proliferation rate, acid tolerance, and enhanced biofilm formation.There were 25 differentially expressed genes related to nutrition metabolism, 20 of which were down-regulated, and 2 of which were related to acid tolerance. [conclusion] simulated weightlessness could induce the phenotype and the corresponding gene changes of Escherichia coli.Among them, the enhancement of biofilm formation ability may pose a potential threat to space flight.
【作者单位】：中国航天员科研训练中心;军事医学科学院微生物所病原微生物生物安全国家重点实验室;
【基金】：“重大新药创制”科技重大专项项目(No.2015ZX09J15102-002) 国家自然科学基金项目(No.31401183)~~
【分类号】：Q937

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