丹参中转录因子SmMYB87基因的克

发布时间：2018-04-14 13:25

本文选题：丹参 + RR　；参考：《中草药》2017年17期

【摘要】：目的克隆丹参Salvia miltiorrhiza中第14亚群R2R3 MYB转录因子基因SmMYB87的全长序列,并对其进行生物信息学和表达分析。方法以丹参总RNA为模板,利用同源克隆和c DNA快速扩增(RACE)技术获得SmMYB87 cDNA全长序列。运用生物信息学软件对该基因及其编码蛋白进行结构和理化性质分析,利用q RT-PCR测定SmMYB87在各器官中的表达并构建融合表达载体pTF-486-SmMYB87分析SmMYB87蛋白的亚细胞定位。结果 SmMYB87基因含有2个内含子和1个732 bp的开放阅读框(ORF),编码243个氨基酸。该基因在根、茎、叶和花中均有表达,且4个器官中的表达量没有显著差异。SmMYB87蛋白在细胞核和细胞膜上均有分布。结论 SmMYB87的序列结构和表达分析为进一步研究其在丹参中的生物学作用奠定了理论基础。
[Abstract]:Objective to clone the full-length SmMYB87 gene of R2R3 MYB transcription factor gene from Salvia miltiorrhiza (Salvia) miltiorrhiza and analyze its expression and bioinformatics.Methods using total RNA of Salvia miltiorrhiza as template, the full-length sequence of SmMYB87 cDNA was obtained by homologous cloning and rapid amplification of C DNA.The structure and physicochemical properties of the gene and its encoded protein were analyzed by bioinformatics software. The expression of SmMYB87 in various organs was determined by Q RT-PCR and the fusion expression vector pTF-486-SmMYB87 was constructed to analyze the subcellular localization of SmMYB87 protein.Results the SmMYB87 gene contained 2 introns and 1 732 BP open reading frame, encoding 243 amino acids.The gene was expressed in root, stem, leaf and flower, and there was no significant difference in the expression of SmMYB87 protein in the nucleus and cell membrane.Conclusion the sequence structure and expression analysis of SmMYB87 lay a theoretical foundation for further study of its biological role in Salvia miltiorrhiza.
【作者单位】：扬州大学生物科学与技术学院;扬州大学园艺与植物保护学院;浙江理工大学生命科学学院;
【基金】：国家自然科学基金资助项目(31700257,81373908) 江苏省高校自然科学研究面上项目(16KJB180032) 中国博士后科学基金面上项目(2015M571826)
【分类号】：S567.53

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