筛选试剂红细胞的血型基因分型方法建立和应用

发布时间：2018-04-15 04:07

  本文选题：红细胞血型 + 试剂红细胞　； 参考：《中国输血杂志》2017年10期

【摘要】：目的建立重要的红细胞血型的基因分型技术用于筛选抗原谱合理的试剂红细胞。方法首先建立检测红细胞血型系统Rh CE、Kidd、MNS、Duffy、Diego、Lutheran、Colton、Lutheran和Kell等的聚合酶链式反应-序列特异性引物(PCR-SSP)分型技术;对于无法获得相关血型试剂的Diego和Colton血型系统,采用测序方法验证该技术检测血型的准确性,其他6个血型系统则用血清学方法验证该技术检测血型的准确性;最后应用该方法对82名O型固定献血者的8个血型系统做基因分型。结果血型基因PCR-SSP分型技术的结果,与血清学和测序检测结果比较,完全一致。所建立的血型基因分型技术对82名O型献血者8种红细胞血型检测,筛选出13位可能适合提供试剂红细胞的献血者。结论成功建立了分型准确、方便、经济的红细胞血型基因PCR-SSP技术,用于从大量献血者中筛选出抗原谱合理的试剂红细胞。
[Abstract]:Objective to establish an important genotyping technique of erythrocyte blood group for screening reagent erythrocytes with reasonable antigen spectrum.Methods the polymerase chain reaction-sequence specific primer (PCR-SSPP) typing technique was established for the detection of erythrocyte blood group system Rh CED MNS Duffy Diegol Lutheran and Kell, and the Diego and Colton blood group systems which could not obtain the relevant blood group reagents were used.The accuracy of this technique was verified by sequencing method, and the other 6 blood group systems were tested by serological method.Finally, 8 blood group systems of 82 O-type fixed blood donors were genotyped by this method.Results the results of PCR-SSP typing were consistent with those of serology and sequencing.The established genotyping technique was used to detect 8 kinds of erythrocyte blood groups of 82 O blood donors and 13 blood donors who might be suitable for providing reagent red blood cells were selected.Conclusion the accurate, convenient and economical PCR-SSP technique of erythrocyte blood group gene has been successfully established, which can be used to screen the red blood cells with reasonable antigen profile from a large number of blood donors.
【作者单位】： 成都军区总医院输血科;中国医学科学院输血研究所;
【基金】：四川省科技厅科技计划项目(2015JY0121)
【分类号】：R457.11
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本文编号：1752437