大豆GmIDD及其同源基因的生物信息学分析及功能预测

发布时间：2018-04-15 19:12

本文选题：大豆 + GmIDD基因　；参考：《大豆科学》2017年04期

【摘要】：利用NCBI和Phytozome数据库进行GmIDD基因的同源基因检索,获取该基因在大豆不同染色体中5个拷贝的序列信息。通过对功能结构域的分析结果显示GmIDD基因5个拷贝均含有2个C2H2型(72~92 aa、114~142 aa)、2个C2HC型(149~169 aa、176~195 aa)锌指结构域。GmIDD基因cDNA序列全长为1 227 bp,编码408个氨基酸。系统进化树分析结果显示GmIDD基因5个拷贝中除了Glyma.14g10940和Glyma.17G228000外,其它IDD蛋白间进化距离较远,表明不同大豆IDD基因在功能上可能存在一定差异。长短日照处理下GmIDD表达量的分析结果显示GmIDD基因受短日照诱导表达,因此推测GmIDD可能参与大豆开花的调控过程。
[Abstract]:The homologous gene of GmIDD gene was searched by NCBI and Phytozome database, and the sequence information of 5 copies of GmIDD gene in different chromosomes of soybean was obtained.The results of functional domain analysis showed that all the 5 copies of GmIDD gene contained 2 C2H2 type 72O9a ~ (92) a ~ (114) ~ ~ (142) a ~ (1) A ~ (2) ~ (2) C2HC type 1499 ~ 169a ~ 166a ~ (176) ~ a ~ (195) a) the cDNA sequence of zinc finger domain. GmIDD gene was 1 227 BP, encoding 408 amino acids.Phylogenetic tree analysis showed that the phylogenetic distance between the other IDD proteins except Glyma.14g10940 and Glyma.17G228000 in the five copies of GmIDD gene was very long, which indicated that there might be some differences in the function of IDD gene in different soybean.The analysis of GmIDD expression under long and short sunlight showed that GmIDD gene was induced by short sunlight, so it was speculated that GmIDD might be involved in the regulation of soybean flowering.
【作者单位】：东北农业大学大豆研究所/大豆生物学教育部重点实验室;
【基金】：黑龙江省普通本科高等学校青年创新人才培养计划(UNPYSCT-2015003) 国家重点研发计划“七大农作物育种”重点专项(2016YFD0101005) 转基因生物新品种培育科技重大专项(2016ZX08004-005) 黑龙江省自然科学基金(C2015009) 东北农业大学“学术骨干”(16XG03) 东北农业大学2016年大学生SIPT计划(201610224141) “十二五”农村领域国家科技计划课题(2013AA102602)
【分类号】：S565.1

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