猪伪狂犬病病毒流行毒株gC基因的分子特征及其对小鼠的毒力试验

发布时间：2018-04-16 10:04

本文选题：伪狂犬病病毒 + 流行毒株　；参考：《中国兽医科学》2017年06期

【摘要】：为了阐明猪伪狂犬病病毒(PRV)流行毒株gC基因的分子特征及其对小鼠的毒力,对来自不同年代分离的6株PRV流行毒株进行了gC基因分子变异特征分析,通过接种BALB/c小鼠检验毒株的毒力。对临床病例分离的6株PRV和30个Gen Bank下载的gC基因序列进行分析表明,这6株PRV与2012年以后流行的毒力增强的变异毒株处于同一分支;这些毒株的氨基酸同源性为98.7%~100%,而与经典的PRV-SC毒株的氨基酸同源性仅为93.2%~94.0%。PRV毒株g C蛋白第52~70位氨基酸为高变区,尤其第64~70位出现7个氨基酸(AAASTPA)插入。对gC蛋白糖基化位点分析表明,PRV变异毒株较经典毒株增加了6个O-连接糖基化位点,且gC蛋白B细胞表位出现3处突变,分别为P69R、P80Q和N83G。gC蛋白上硫酸乙酰肝素结合域(HBD)出现2处突变,分别为HBD1第5位氨基酸由经典毒株的P突变为Q,HBD3的第3位氨基酸由Y突变为C。用分离的6株PRV接种BALB/c小鼠试验显示,PRV-HRB和PRV-SH毒株半数致死量(LD50)均为1×10~(3.21)/mL,PRV-DL、PRV-JL及PRV-GZL的LD50为1×10~(3.75)/mL,PRV-JF的LD50为1×10~(4.32)/mL。上述结果阐明了PRV流行毒株g C基因的分子特征及其对小鼠的毒力,为该病毒的致病机制研究提供了科学依据。
[Abstract]:In order to elucidate the molecular characteristics of GC gene of porcine pseudorabies virus (PRV) epidemic strain and its virulence to mice, the molecular variation characteristics of GC gene in 6 strains of PRV epidemic virus isolated from different years were analyzed.The virulence of the strain was tested by inoculating BALB/c mice.The analysis of 6 PRV strains and 30 Gen Bank downloaded GC gene sequences showed that the 6 PRV strains were in the same branch as the virulence mutant strains that were prevalent after 2012.The amino acid homology of these strains was 98.7 and 100, while the amino acid homology of the classical PRV-SC strains was that the amino acids at the 52G C protein of the 93.2%~94.0%.PRV strain were hypervariable, especially 7 amino acids were inserted at the 64th and 70th position.The analysis of glycosylation sites of GC protein showed that the mutant strains of PRV increased 6 O- linked glycosylation sites compared with the classical strains, and there were 3 mutations in the epitopes of GC protein B cells.There were two mutations in heparin sulfate binding domain (HBDs) of P69RG P80Q and N83G.gC protein, respectively. The amino acid at the fifth position of HBD1 was mutated from P of the classical strain to the third amino acid of QHbd3 from Y to C.BALB/c mice inoculated with 6 isolated PRV strains showed that the LD50 of PRV-HRB and PRV-SH strain were 1 脳 10 ~ (-1) / m ~ (-1) / m ~ (-1) and 1 脳 10 ~ (75) / m ~ (-1) / m ~ (-1) LD50 of PRV-JF and 1 脳 10 ~ (10) / m ~ (-1) / m ~ (-1) of PRV-JF, respectively, and the LD50 of PRV-GZL was 1 脳 10 ~ (75) / m ~ (-1) / m ~ (-1) of PRV-JF.The above results illustrate the molecular characteristics of the G C gene of PRV and its virulence to mice, which provides a scientific basis for the study of the pathogenicity of the virus.
【作者单位】：中国农业科学院哈尔滨兽医研究所兽医生物技术国家重点实验室;
【基金】：黑龙江省自然科学基金项目(C2015064) 哈尔滨市应用技术研究和开发项目(2015RAQYJ066)
【分类号】：S852.651

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