糖尿病大鼠Nfic与成骨相关基因表达的研究

发布时间：2018-04-16 10:07

  本文选题：糖尿病 + 成骨细胞　； 参考：《中华老年口腔医学杂志》2016年02期

【摘要】：目的:探究糖尿病大鼠Nfic的表达状况及其与其他成骨相关基因表达改变。方法:6周龄Wistar雄性大鼠30只,随机分为对照组和糖尿病模型组。模型组大鼠腹腔注射链脲佐菌素(65mg/kg)建立1型糖尿病模型。观察两组大鼠体重、血糖变化;模型建成后8周处死大鼠,Q-PCR法检测双侧股骨Nfic、Runx2、OSX(Oster ix)、COL2A1(Ⅰ型胶原)、OC(骨钙素)、ALP、BMP-2、IGF-1(胰岛素样生长因子)的mRNA表达水平。结果:8周后,模型组大鼠体重降低或保持不变,对照组大鼠体重持续稳定增长;模型组血糖持续保持在高血糖水平,对照组血糖位于正常范围;Q-PCR检测发现,Nfic、Runx2、OSX、COL2A1、OC、IGF-1、BMP-2基因mR NA表达中,模型组明显低于对照组。ALP基因的mRNA表达中,模型组显著高于对照组(P0.01)。结论:1型糖尿病大鼠的高糖状态使得Nfic等成骨相关基因表达下降,成骨标记产物降低。
[Abstract]:Aim: to investigate the expression of Nfic and other osteoblast related genes in diabetic rats.Methods 30 Wistar male rats aged 6 weeks were randomly divided into control group and diabetic model group.Type 1 diabetic model was established by intraperitoneal injection of streptozotocin (65 mg / kg) in the model group.The changes of body weight and blood glucose in the two groups were observed, and the expression of mRNA in the bilateral femur of NficCon Runx2OSX Oster Oster Oster OC-1 (type 鈪，

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