青藏高原5种害鼠vkorc1基因的测序分析

发布时间：2018-04-16 11:14

本文选题：抗凝血杀鼠剂抗药性 + vkorc　；参考：《草业科学》2016年06期

【摘要】：维生素K环氧化物还原酶复合物1基因(vkorc1)的变异是导致鼠类对抗凝血杀鼠剂产生抗性的主要原因。本研究利用转录组测序的方法,分析青藏高原地区5种主要害鼠——高原鼢鼠(Eospalax baileyi)、高原鼠兔(Ochotona curzoniae)、长尾仓鼠(Cricetulus longicaudatus)、青海田鼠(Lasiopodomys fuscus)和喜马拉雅旱獭(Marmota himalayana)的vkorc1基因序列信息。同时,采集5个种群共54只高原鼢鼠,对vkorc1基因全序列进行测序分析。结果显示,从转录组组装结果中成功获得5种动物的vkorc1基因编码区全序列,其中青海田鼠、长尾仓鼠、高原鼢鼠vkorc1基因编码区长度为486bp,喜马拉雅旱獭和高原鼠兔为492bp;在DNA序列水平上,5种高原动物存在143个变异位点,与大鼠的序列相似性为80.1%~90.7%,而与小鼠的序列相似性为79.7%~89.8%。在氨基酸序列水平上,5种高原动物存在37个变异位点,大鼠的序列相似性为84.0%~92.0%,而与小鼠的序列相似性为85.9%~92.0%;未发现与已知抗凝血杀鼠剂抗性相关的氨基酸位点。对5个种群54只高原鼢鼠vkorc1基因全序列的测序分析显示,比对后的全长为1 808bp,共检测到8个变异位点,其中两个为插入缺失位点,全部变异都发生在内含子区。本研究首次以基因序列为分析对象,可以为青藏高原地区的鼠害防治提供关键基础资料。
[Abstract]:The variation of vitamin K epoxide reductase complex 1 gene (vkorc1) is the main cause of resistance to anticoagulant rodenticide in mice.Using transcriptome sequencing method, the vkorc1 gene sequences of five major rodent species, Eospalax Baileyii, Ochotona curzoniae, Cricetulus longicaudatusus, Lasiopodomys fuscusae and Marmohimta alayana, were analyzed in Qinghai-Tibet Plateau.At the same time, 54 plateau zokors were collected from 5 populations and sequenced the vkorc1 gene.The results showed that the complete sequence of vkorc1 gene coding region of 5 species of animals was successfully obtained from the results of transcriptome assembly, including Qinghai vole, long-tailed hamster.The length of vkorc1 gene coding region of plateau zokor was 486 BP, and that of Himalayan marmot and plateau pika was 492 BP. There were 143 mutation sites in 5 species of plateau animals at the DNA sequence level, the similarity with the sequence of rats was 80.1 BP and that with mice was 79.7% and 89.8 respectively.At the amino acid sequence level, there were 37 mutation sites in 5 species of plateau animals, the sequence similarity of rats was 84.0 and that of mice was 85.90.There was no amino acid locus associated with known resistance to anticoagulant rodenticide.The whole vkorc1 gene sequence of 54 plateau zokors from 5 populations was analyzed. The total length of the vkorc1 gene was 1 808bp. eight mutation sites were detected, two of which were insertion deletion sites, all of which occurred in intron region.In this study, the gene sequence was first used as the analysis object, which can provide the key basic data for rodent control in Qinghai-Xizang Plateau.
【作者单位】：中国科学院西北高原生物研究所;中国科学院大学;潍坊滨海开发区管委会;青海省畜牧兽医科学院;
【基金】：青海省科技支撑计划项目——青藏高原草地鼠害综合防控技术集成与示范(2014-NS-113) 青海省科技项目——贵南县退化草地恢复治理及生态畜牧业关键技术集成与应用(2014-NS-118)
【分类号】：S812.6

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