刺五加鲨烯环氧酶基因DNA和启动子的克隆与分析

发布时间：2018-04-17 20:03

本文选题：刺五加 + SE基因　；参考：《基因组学与应用生物学》2017年09期

【摘要】：本研究以刺五加鲨烯环氧酶(squalene epoxidase,SE)基因的c DNA序列为基础设计特异性引物,利用PCR和TAIL-PCR扩增SE DNA的全长序列,结合Plant CARE等软件,对启动子中的作用元件进行生物信息学分析。克隆得到长6 307 bp的刺五加SE DNA和启动子序列,包含8段外显子、7段内含子及两端的非翻译区,编码554个氨基酸;上游启动子序列长1 907 bp,含36个TATA-box,27个CAAT-box,此外还有茉莉酸甲酯调控元件、脱落酸调控元件以及光调控元件等多种顺势作用元件,表明SE基因表达受植物激素、光照以及温度等多种因素的调控。
[Abstract]:Based on the c DNA sequence of squalene epoxidase (SEE) gene of Acanthopanax senticosus, a specific primer was designed to amplify the full-length sequence of SE DNA by PCR and TAIL-PCR. Combined with Plant CARE and other software, bioinformatics analysis was carried out on the acting elements in the promoter.A 6 307bp long SE DNA and promoter sequence of Acanthopanax senticosus were cloned, which contained 8 exons and 7 introns and untranslated regions at both ends, encoding 554 amino acids.The upstream promoter contains 36 TATA-boxboxes, 27 CAAT-boxs, methyl jasmonate regulatory elements, abscisic acid regulatory elements and photoregulatory elements, indicating that SE gene expression is affected by plant hormones.Light and temperature and other factors of regulation.
【作者单位】：华北理工大学生命科学学院;
【基金】：国家自然科学基金项目(31570683) 河北省教育厅资助科研项目(QN2014102) 华北理工大学培育基金(SP201508);华北理工大学大学生创新创业训练计划(X2016045)共同资助河北省大学生创新创业训练计划(201610081045)
【分类号】：Q943.2;S567.19

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