福建省漳州市2013-2015年流行麻疹病毒基因型别鉴定及基因特征分析

发布时间：2018-04-17 21:25

本文选题：麻疹病毒 + 核蛋白基因　；参考：《中国疫苗和免疫》2017年02期

【摘要】：目的了解2013-2015年漳州市麻疹病毒基因型流行情况。方法用Real-time RT-PCR法对2013-2015年漳州市的392份疑似麻疹病例咽拭子标本进行核酸检测;用RT-PCR法扩增核酸阳性标本的N基因羧基末端594个核苷酸片段,并对扩增产物测序;以N基因羧基末端450个核苷酸序列构建系统进化树,并进行核苷酸和氨基酸同源性分析。结果 28份标本麻疹病毒核酸阳性,阳性率7.1%(28/392);获得21份麻疹病毒的核苷酸序列,经基因型鉴定18株为H1a基因亚型,1株为A基因型,2株为D8基因型。漳州市所有的H1a亚型麻疹病毒与H1a亚型参考株China93-2-AF045205.1-H1a之间核苷酸和氨基酸同源性分别为98.0%-99.1%和97.3%-99.3%。A基因型麻疹病毒与疫苗株Shanghai-191-DQ390225-A的核苷酸和氨基酸同源性分别为99.6%和99.3%。2株D8基因型麻疹病毒之间核苷酸和氨基酸同源性均为100%,它们与世界卫生组织(WHO)参考株MVi/Manchester.GBR/30.94-AF280803-D8的核苷酸和氨基酸同源性分别为96.4%和96.7%。结论 2013-2015年漳州市麻疹病毒优势基因型为H1a基因亚型;A基因型为疫苗相关株;漳州市首次出现D8基因型麻疹病毒输入性病例。
[Abstract]:Objective to investigate the prevalence of measles virus genotypes in Zhangzhou City from 2013 to 2015.Methods the nucleic acid was detected by Real-time RT-PCR method in 392 suspected measles specimens from Zhangzhou from 2013 to 2015, and 594 nucleotide fragments of N gene carboxyl terminal of nucleic acid positive samples were amplified by RT-PCR method, and the amplified products were sequenced.Phylogenetic tree was constructed from 450 nucleotide sequences of carboxyl terminal of N gene, and nucleotide and amino acid homology were analyzed.Results the nucleic acid of measles virus was positive in 28 samples, the positive rate was 7.1 / 392.The nucleotide sequences of 21 measles viruses were obtained, and 18 strains were identified as H1 a gene subtype and 1 strain as genotype A and 2 strains as D8 genotype.The nucleotide and amino acid homology between all HIVa subtype measles virus and China93-2-AF045205.1-H1a is 98.0-99.1% and 97.3-99.3.A respectively. The nucleotide and amino acid homology between measles virus genotype and vaccine strain Shanghai-191-DQ390225-A is 99.6% and 99.3.2%, respectively.The nucleotide and amino acid homology between genotypes of measles virus was 100. The nucleotide and amino acid homology of the nucleotide and amino acid homology between the genotype measles virus and World Health Organization (WHO) reference strain MVi/Manchester.GBR/30.94-AF280803-D8 were 96. 4% and 96. 7%, respectively.Conclusion the predominant genotype of measles virus in Zhangzhou City from 2013 to 2015 is H1 a gene subtype A genotype which is vaccine related strain and the first case of measles virus imported with D8 genotype in Zhangzhou City.
【作者单位】：漳州市疾病预防控制中心;
【分类号】：R511.1

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