番茄与马铃薯TIFY家族基因的鉴定与比较分析

发布时间：2018-04-17 21:55

  本文选题：番茄 + TIFY家族　； 参考：《分子植物育种》2017年04期

【摘要】：TIFY蛋白是一类植物特有的转录因子,在植物的生长发育、信号传导以及胁迫反应中起重要作用。尽管一些模式植物TIFY家族基因已经被鉴定,但在茄科植物中有关TIFY家族基因的研究却很少。本研究利用生物信息学手段分别从番茄与马铃薯中挖掘到21个Sl TIFY和21个St TIFY基因,并系统性分析这些TIFY基因的序列、表达以及进化特性。染色体定位与扩增模式分析显示,番茄和马铃薯的TIFY基因散落分布在不同染色体上,家族成员扩增主要由串联和片段重复产生。序列分析表明:番茄TIFY基因结构被分为12种,马铃薯TIFY基因结构被分为8种;番茄和马铃薯TIFY蛋白共享了保守基序1,其它保守基序显示不同程度基因特异性,保守基序组成模式表现出高度多样化。系统进化分析显示,番茄和马铃薯TIFY蛋白被分为7大类群,同类群内结构与序列相似性较高。选择压力检测表明,番茄的10对同源基因和马铃薯的19对同源基因均受到负选择作用。表达谱结果表明,番茄和马铃薯TIFY基因的表达呈现较高的多样性,这可能与多样化的功能相关。这些研究结果为功能解析番茄和马铃薯TIFY基因提供理论指导。
[Abstract]:TIFY protein is a kind of plant specific transcription factor, which plays an important role in plant growth and development, signal transduction and stress response.Although some model plant TIFY family genes have been identified, few studies on TIFY family genes have been carried out in eggplant plants.In this study, 21 SL TIFY and 21 St TIFY genes were extracted from tomato and potato by bioinformatics, and the sequence, expression and evolution of these TIFY genes were analyzed systematically.Chromosome localization and amplification pattern analysis showed that the TIFY genes of tomato and potato were scattered on different chromosomes and the family members were mainly produced by tandem and fragment repetition.Sequence analysis showed that tomato TIFY gene structure was divided into 12 species, potato TIFY gene structure was divided into 8 species, tomato and potato TIFY protein shared conserved motif 1, other conserved motifs showed different degree of gene specificity.The conserved motif composition model is highly diversified.Phylogenetic analysis showed that the TIFY protein of tomato and potato were divided into 7 groups, and the structure and sequence of the same group were similar.The selection pressure test showed that 10 pairs of homologous genes of tomato and 19 pairs of homologous genes of potato were negatively selected.The results of expression profile showed that the expression of TIFY gene in tomato and potato showed high diversity, which may be related to the diversity of function.These results provide theoretical guidance for functional analysis of TIFY gene in tomato and potato.
【作者单位】： 周口师范学院生命科学与农学学院;周口师范学院河南省作物分子育种与生物反应器重点实验室;
【基金】：河南省教育厅项目(17B180009) 周口师范学院2014年高层次人才科研启动经费资助项目(ZKNU2014109) 周口师范学院2015年度校本项目(ZKNUB215103)共同资助
【分类号】：S532;S641.2
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本文编号：1765438