光控和埋置褪黑激素促进绒山羊长绒的皮肤毛囊差异表达基因网络分析

发布时间：2018-04-18 10:20

本文选题：绒山羊 + 光控增绒　；参考：《中国农业大学学报》2017年09期

【摘要】：为研究光控增绒和埋置褪黑激素促进绒山羊在非产绒季节(5—8月份)长绒的皮肤毛囊差异表达基因之间的互作关系,并筛选出皮肤毛囊发育相关的重要功能基因,以Agilent绵羊的全基因组表达谱芯片杂交获得的光控增绒羊皮肤差异表达的99个基因及埋置褪黑激素绒山羊皮肤差异表达的83个基因作为研究对象,结合NCBI数据库,利用RStudio程序包和Cytoscape 3.3.0构建差异基因网络并分析。光控增绒试验组差异基因构建了1个主网络和4个小网络;对照组差异基因构建了1个主网络和5个小网络。与表达量结合分析基因网络发现,K25、KAP16-2基因上调促进ISG17、RSRP1、GIMAP1和CYTIP基因的下调,间接促进TNFAIP6基因的下调;CYP17A1、V15和K2-12(K85)基因的上调促进ddit3基因的下调;KAP16-3基因促进CYP17A1基因的同时抑制CYP1A1基因。埋置褪黑激素试验组差异基因构建了2个主网络和7个小网络,对照组差异基因构建了3个主网络和1个小网络。与表达量结合分析基因网络发现,CTNNB1基因的上调促进COL3A1基因的下调;FLT-1基因的上调促进CGA基因的上调和COL1A2基因的下调;PAX6基因的上调促进DOK4基因的上调和LOC101110099基因的下调;P450基因的上调促进BLG基因的下调。光控增绒羊皮肤中筛选出来的基因中角蛋白、角蛋白关联蛋白、CYP17A1、SP-D等23个显著上调基因和CYP1A1、COL6A5、LOC100101238、ddit3等13个显著下调基因对光控增绒羊皮肤毛囊提前进入兴盛期有重要作用;埋置褪黑激素的绒山羊皮肤中筛选出来的基因中CTNNB1、PAX6、DOK4、LOC105602529等13个显著上调基因和酪蛋白家族、胶原蛋白类、LOC101110099等12个显著下调基因之间的信号传导增强,从而促进绒山羊绒毛进入兴盛期。
[Abstract]:In order to study the interaction between the differentially expressed genes in the skin follicles of cashmere goats during the non-cashmere season in May to August, the important functional genes related to the development of the hair follicles were screened out in order to study the interaction between the light-controlled cashmere enhancement and the embedding of melatonin.Ninety-nine differentially expressed genes in the skin of light-controlled cashmere sheep and 83 differentially expressed genes in the skin of buried melatonin goat were used as the research objects, and the NCBI database was used.RStudio package and Cytoscape 3.3.0 were used to construct and analyze the differential gene network.One main network and four small networks were constructed in the experiment group, and one main network and five small networks were constructed in the control group.The up-regulation of KAP16-2 gene promoted the down-regulation of ISG17RSRP1GIMAP1 and CYTIP gene, and indirectly promoted the down-regulation of TNFAIP6 gene (CYP17A1V15 and K2-12K85) gene. The up-regulation of KAP16-3 gene promoted the downregulation of ddit3 gene and inhibited CYP1A1 gene at the same time.Two main networks and seven small networks were constructed in melatonin test group, and 3 main networks and 1 small network were constructed in control group.Expression binding Analysis Gene Network found that the up-regulation of CTNNB1 gene promoted the down-regulation of COL3A1 gene and the up-regulation of FLT-1 gene promoted the up-regulation of CGA gene and the down-regulation of COL1A2 gene PAX6 gene promoted the up-regulation of DOK4 gene and the down-regulation of LOC101110099 gene.The up-regulation of P450 gene promotes the down-regulation of BLG gene.There were 23 up-regulated genes such as keratin, CYP17A1SP-D and 13 down-regulated genes such as CYP17A1SP-D and 13 down-regulated genes such as CYP1A1, COL6A5, LOC100101238, DDIT3, etc., which played an important role in the early development of hair follicles of light-controlled cashmere sheep.13 genes, such as CTNNB1, PAX6, DOK4, LOC105602529 and so on, significantly up-regulated the signal transduction among 12 down-regulated genes, such as collagen, LOC101110099 and so on, among the genes screened out from the skin of cashmere goats embedded with melatonin, such as CTNNB1, PAX6, DOK4, LOC105602529 and so on.Thus promoting cashmere goat villus into the prosperous period.
【作者单位】：内蒙古农业大学动物科学学院;内蒙古农牧业科学院畜牧研究所;中国科学院昆明动物研究所;鄂托克前旗兽医局;
【基金】：国家自然科学基金资助项目(31260545);国家自然科学基金项目(31402052) 国家公益性行业(农业)科研专项(201303059) 内蒙古自治区自然科学基金资助项目(2014BS0333) 内蒙古自治区创新基金项目(2013CXJJM03) 内蒙古自治区科技创新引导项目内蒙古自然科学基金博士项目(2014BS0313) 内蒙古民族大学科学研究项目(BS308)
【分类号】：S827

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