大肠杆菌热休克或冷休克启动子调控的基因表达载体

发布时间：2018-04-18 13:04

本文选题：生物活性蛋白 + 分子伴侣　；参考：《微生物学通报》2017年06期

【摘要】：基因重组技术已经成为获得各种酶和生物活性蛋白的主要手段。虽然很多基因已在大肠杆菌中得到高效表达,但是当人们认定某种蛋白对科学研究或生产应用极为重要时,却常常因为其基因表达水平很低或产生包涵体而感到束手无策。表达载体pHsh和pEXC通过激活热休克或冷休克转录调控机制提高分子伴侣的表达水平,从而降低目标蛋白的细胞毒性并减少包涵体形成。应用于生物合成、分子修饰或生物降解的高温酶可以通过pHsh系统表达获得高产,而科研和诊疗所需要的来源于动植物和常温微生物的基因可以通过pEXC系统获得高效表达。这些新载体的发展为重组蛋白的小规模制备和大规模生产提供了新策略和有效途径。
[Abstract]:Recombinant DNA technology has become the main means of access to a variety of enzymes and bioactive proteins. Although many genes have been expressed in Escherichia coli, but when people identified a protein of scientific research or production application is extremely important, but often because the expression level is low or have inclusions and feel at a loss what to do. The expression vector pHsh and pEXC through the activation of heat shock or cold shock transcriptional regulation mechanism to improve the expression level of molecular chaperones, thereby reducing the cytotoxicity of target protein and reduce the formation of inclusion body. Used in high temperature enzyme biosynthesis, molecular modification or biodegradation can obtain high yield by pHsh expression system, and the source of scientific research and clinic needs in plants room temperature and microbial gene can be highly expressed by pEXC system. The development of these new carriers for the small scale preparation of recombinant protein New strategies and effective ways are provided for mass production.

【作者单位】：江苏大学环境与安全工程学院生物质能源研究所;
【基金】：国家自然科学基金项目(No.30970062) 江苏省重点研发计划(现代农业)项目(No.BE2016353) 江苏省自然科学基金青年基金项目(No.BK20160502) 江苏大学高级人才科研启动基金项目(No.10JDG117,16JDG031)~~
【分类号】：Q78

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