尿路上皮癌BRCA1基因与蛋白表达的相关性

发布时间：2018-04-18 22:35

  本文选题：尿路上皮癌 + 乳腺癌易感基因　； 参考：《山东大学学报(医学版)》2017年05期

【摘要】：目的探讨尿路上皮癌乳腺癌易感基因1(BRCA1)mRNA水平与蛋白表达的相关性。方法收集50例尿路上皮癌患者标本组织进行石蜡切片3~5μm,采用免疫组织化学法检测BRCA1基因蛋白的表达。通过提取石蜡切片组织RNA进行反转录,采用实时定量PCR法检测BRCA1基因mRNA的表达。结果 BRCA1 mRNA低表达率64%,中表达率36%;BRCAl免疫组织化学染色阳性率为88%,阴性率为12%。采用SSPS 17.0统计学软件,经Wilcoxon两样本检验,BRCAl mRNA低表达组与中表达组的免疫组化染色程度差异有统计学意义(P0.000 1);Spearman等级相关分析显示两者呈正相关性(rs=0.652 9)。结论实时荧光定量PCR检测mRNA表达与免疫组化检测蛋白表达皆为评估尿路上皮癌的可选方法。
[Abstract]:Objective to investigate the correlation between 1(BRCA1)mRNA level and protein expression of breast cancer in urothelial carcinoma.Methods paraffin sections of 35 渭 m were collected from 50 patients with urothelial carcinoma and the expression of BRCA1 gene was detected by immunohistochemistry.The expression of BRCA1 gene mRNA was detected by real-time quantitative PCR method by extracting RNA from paraffin sections for reverse transcription.Results the low expression rate of BRCA1 mRNA was 64%, the middle expression rate was 36%. The positive rate of immunohistochemical staining of BRCAl was 88%, and the negative rate was 12%.Using SSPS 17.0 statistical software, two samples of Wilcoxon were used to test the difference in the degree of immunohistochemical staining between the low expression group of BRCAl mRNA and the middle expression group. There was a significant difference in the degree of immunohistochemical staining between the two groups. The correlation analysis showed that there was a positive correlation between the two groups.Conclusion Detection of mRNA expression by real-time fluorescence quantitative PCR and expression of protein by immunohistochemistry are the alternative methods for the evaluation of urothelial carcinoma.
【作者单位】： 山东大学齐鲁医院泌尿外科;
【分类号】：R737.1
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本文编号：1770412