不同果袋对‘阳光玫瑰’葡萄香气组分及合成相关基因表达的影响

发布时间：2018-04-19 02:24

本文选题：葡萄 + 套袋　；参考：《果树学报》2017年01期

【摘要】：【目的】探讨不同类型果袋对‘阳光玫瑰’葡萄香气组分及合成相关基因表达的影响,为葡萄生产栽培选择适宜的果袋提供理论指导。【方法】以‘阳光玫瑰’葡萄为材料,套普通单层白纸袋、透明微孔塑料袋、双层外黄内黑纸袋和单层绿纸袋4种不同果袋,以普通单层白纸袋为对照(以下简称白袋、透明袋、黑袋和绿袋)。采用实时荧光定量PCR法分析脱氧木酮糖磷酸合酶基因(DXS3)、香叶基二磷酸合酶基因(GPPS)、里那醇合成酶基因(LIS)的表达变化,利用GC-MS技术测定葡萄香气组分。【结果】与对照相比,套透明袋果实较大,可滴定酸含量偏低,但可溶性固形物含量高,糖酸比高,差异显著;套黑纸袋和绿袋果实可溶性固形物含量较低。套透明袋果实中单萜类物质特征香气里那醇含量丰富,香叶醇、橙花醇、萜品醇等辅助类香气含量也很高,玫瑰香气浓郁;而套黑袋果实则特征香气含量最少;套绿袋果实香气物质种类少。套透明袋果实DXS3、GPPS和LIS基因表达量上调,促进单萜物质生成,单萜物质含量丰富,而套黑袋抑制了基因表达,推迟了基因表达高峰,影响单萜物质生成。套绿袋果实和对照相比基因表达量没有显著性差异。【结论】套不同的果袋对‘阳光玫瑰’葡萄果实香气组分及合成相关基因表达量的变化有显著的差异。
[Abstract]:[objective] to investigate the effects of different fruit bags on aroma components and gene expression of 'Sunshine Rose' grape, and to provide theoretical guidance for the selection of suitable fruit bags for grape production and cultivation. [methods] 'Sunshine Rose' grape was used as material.There are four kinds of fruit bags: ordinary single white paper bag, transparent microporous plastic bag, double outer yellow inner black paper bag and single layer green paper bag, which are compared with ordinary single layer white paper bag (white bag, transparent bag, black bag and green bag).The expression of deoxyxyxylglycosylphosphatase (DXS3), germplasm diphosphate synthase gene (GPPSG) and linatol synthase gene (LISs) were analyzed by real-time fluorescence quantitative PCR. The aroma components of grape were determined by GC-MS technique. [results] compared with the control,The content of soluble solids was higher, the ratio of sugar to acid was higher, and the contents of soluble solids in black paper bags and green bags were lower than those in black paper bags and green bags.The characteristic aroma of monoterpenes was abundant in the fruit of transparent bag, the contents of geraniol, hesperol and terpene alcohol were also very high, and the aroma of rose was strong, but the characteristic aroma of black bag fruit was the least.There are few kinds of aroma substances in green bag fruit.The expression of DXS3GPPS and LIS genes was upregulated, which promoted the formation of monoterpenes, which was rich in monoterpenes, while the black bag inhibited the gene expression, delayed the peak of gene expression, and affected the production of monoterpenes.There was no significant difference in gene expression between green bag fruit and control group. [conclusion] there were significant differences in aroma components and synthesis related gene expression in fruit of 'Sunshine Rose' grape with different fruit bags.
【作者单位】：南京农业大学园艺学院;
【基金】：国家现代农业葡萄产业技术体系(CARS-30) 国家948重点项目(2016-X19)
【分类号】：S663.1

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