玉米光敏色素基因C的克隆与苗期光暗条件下表达分析

发布时间：2018-04-19 05:26

本文选题：玉米 + 光敏色素C　；参考：《分子植物育种》2017年01期

【摘要】：光敏色素在植物的种子萌发、幼苗去黄化、叶片开张、茎伸长、开花等发育过程中具有重要调节作用。本研究根据玉米基因组数据库设计引物,获得ZmPHYC1和ZmPHYC2基因序列,分析其编码的氨基酸序列和结构域发现,6个不同光温敏感性玉米品种中光敏色素C基因序列未出现大片段的序列插入或缺失,仅发现少量SNP;ZmPHYC1与ZmPHYC2蛋白中含有1个GAF结构域、1个Phytochrome结构域、2个PAS结构域、1个His Kinase A结构域和1个类似组氨酸激酶的ATP激酶结构域;遗传进化树分析表明,单子叶和双子叶植物明显聚为不同的类别,并且玉米和高粱之间的同源性要高于玉米中PHYC1与PHYC2之间的同源性。利用实时荧光定量PCR技术分析ZmPHYC1和ZmPHYC2的表达模式发现,在光暗处理条件下,两个基因在不同品种间及各组织部位中表达量无显著差异。
[Abstract]:Guang Min pigment plays an important role in the process of seed germination, seedling desalination, leaf opening, stem elongation, flowering and so on.In this study, ZmPHYC1 and ZmPHYC2 gene sequences were obtained by designing primers based on maize genome database.By analyzing the amino acid sequence and domain, we found that there was no insertion or deletion of large fragment of Guang Min pigment C gene in 6 maize varieties with different light and temperature sensitivity.Only a few SNPs ZmPHYC1 and ZmPHYC2 proteins contained one GAF domain, one Phytochrome domain, two PAS domains, one His Kinase A domain and one ATP kinase domain similar to histidine kinase.Monocotyledon and dicotyledonous plants were clustered into different classes, and the homology between maize and sorghum was higher than that between PHYC1 and PHYC2 in maize.The expression patterns of ZmPHYC1 and ZmPHYC2 were analyzed by real-time fluorescence quantitative PCR. It was found that there was no significant difference in the expression of ZmPHYC1 and ZmPHYC2 between different cultivars and tissues under the condition of light and dark treatment.
【作者单位】：沈阳农业大学;辽宁省农业科学院;
【基金】：辽宁省教育厅重点项目(LSNZD201608) 辽宁省自然基金(2014027027) 辽宁省科技厅农业攻关及产业化项目(2015103001)共同资助
【分类号】：Q943.2;S513

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