水稻脆秆褐穗突变体fb1的鉴定与基因定位

发布时间：2018-04-19 18:54

本文选题：水稻 + 褐穗　；参考：《核农学报》2017年12期

【摘要】：为鉴定新的脆杆突变体,从籼型水稻恢复系缙恢10号的甲基磺酸乙酯(EMS)诱变体库中鉴定了一个全生育期植株变脆且开花后稻穗呈褐色的突变体,暂命名为脆秆褐穗突变体fb1,对其进行了机械强度、光合色素含量、木质素和纤维素含量等测定,并开展了遗传分析和基因定位等研究。结果表明,与野生型相比,fb1茎秆和叶片的最大载荷分别下降了43.3%和63.1%,茎秆的纤维素和木质素含量分别为野生型的76.5%和66.6%,叶片的纤维素和木质素含量则分别为野生型的91.0%和95.5%,均显著降低。开花前,fb1的穗部性状与野生型相比无变化,开花后颖壳逐渐褐化,成熟后呈灰褐色。fb1的叶绿素a、叶绿素b和类胡萝卜素含量分别为野生型的74.8%、90.5%和85.3%,均呈显著或极显著下降。遗传分析表明,该性状受一对隐性核基因调控,基于西农1A/fb1的F2群体,利用SSR等分子标记最终将调控基因FB1精细定位在第1染色体SSR标记RM1268和RM11669之间42 kb的物理范围内,包含7个注释基因。该研究结果不仅为FB1基因的图位克隆奠定了基础,也为植物细胞壁发育分子机理研究和新能源水稻育种提供了材料。
[Abstract]:In order to identify a new brittleness mutant, a new mutant with brown spikes after flowering was identified from the mutant bank of Jinhui 10, an indica rice restorer line Jinhui 10. The mutant fb1 was tentatively named as Fb1. The mechanical strength, photosynthetic pigment content, lignin and cellulose content were determined, and genetic analysis and gene mapping were carried out. The results show that Compared with wild type, the maximum load of stem and leaf decreased by 43.3% and 63.1%, respectively. The contents of cellulose and lignin of stem were 76.5% and 66.6% of that of wild type, respectively, and the contents of cellulose and lignin in leaves were 91.0% and 95.5B of wild type, respectively. The panicle characters of Fb1 before anthesis had no change compared with the wild type. The glume was browning gradually after anthesis, and the content of chlorophyll a, chlorophyll b and carotenoid were 74.80.5% and 85.3% of that of the wild type, respectively, and the contents of chlorophyll b and carotenoid were significantly or extremely significantly lower than those of the wild type, and the contents of chlorophyll a, chlorophyll b and carotenoid were 74.80.5% and 85.3% of the wild type, respectively. Genetic analysis showed that the trait was regulated by a pair of recessive nuclear genes. Based on the F2 population of Xinong 1A/fb1, the regulatory gene FB1 was finely mapped within the physical range of 42 kb between RM1268 and RM11669 on chromosome 1 by using SSR and other molecular markers. Contains seven annotated genes. The results not only laid a foundation for the mapping cloning of FB1 gene, but also provided materials for the molecular mechanism of cell wall development in plants and the breeding of rice with new energy sources.
【作者单位】：西南大学水稻研究所/转基因植物与安全控制重庆市重点实验室;四川省农业科学院水稻高粱研究所;
【基金】：国家自然科学基金(31171178) 中央高校基本科研业务费项目(XDJK2015C117)
【分类号】：S511

【相似文献】