超声靶向微泡破坏联合脂质体介导pSilencer 3.1-SATB1基因转染前列腺癌细胞效果观察

发布时间：2018-04-20 06:01

本文选题：超声靶向微泡破坏 + 基因转染　；参考：《山东医药》2017年30期

【摘要】：目的探讨超声靶向微泡破坏(UTMD)联合脂质体介导的沉默特异性核基质蛋白1基因(pSilencer3.1-SATB1)对人前列腺癌细胞转染的效果。方法体外培养人前列腺癌雄激素非依赖型细胞株DU145,分为对照组、微泡组、超声组、超声+微泡组、脂质体组、超声+微泡+脂质体组。微泡组加入适量微泡,超声组仅给予超声辐照,超声+微泡组加入微泡后予以超声辐照,脂质体组加入脂质体,超声+微泡+脂质体组加入微泡、脂质体后予以超声辐照,对照组不做任何处理。24 h后采用流式细胞仪观察各组增强型绿色荧光蛋白(EGFP)表达,并检测EGFP荧光细胞的比例,计算基因转染率以评价转染效果。结果荧光显微镜下,超声+微泡+脂质体组可见大量EGFP表达,明显多于其他各组。对照组、微泡组、超声组、超声+微泡组、脂质体组、超声+微泡+脂质体组基因转染率分别为0.18%±0.09%、0.25%±0.11%、1.14%±0.13%、2.96%±0.16%、3.99%±0.12%、7.16%±0.17%,超声+微泡+脂质体组的基因转染率高于其他各组(P均0.05)。结论 UTMD可增强脂质体介导质粒pSilencer3.1-SATB1对DU145细胞的转染效果,提高转染率,为前列腺癌治疗的研究提供了一个新的途径。
[Abstract]:Objective to investigate the transfection of human prostate cancer cells by ultrasound targeting microbubble destruction UTMD and liposome mediated silencing specific nuclear matrix protein-1 gene pSilencer3.1-SATB1. Methods Human prostate cancer androgen independent cell line DU145 was cultured in vitro and divided into control group, microbubble group, ultrasound group, ultrasonic microbubble group, liposome group and ultrasound microbubble liposome group. Appropriate amount of microbubbles were added in the microbubble group, ultrasound was used only in the ultrasonic group, ultrasound irradiation was given in the ultrasonic microbubble group, liposome was added in the liposome group, microbubble was added in the ultrasonic microbubble liposome group, the liposome was then irradiated by ultrasound. Flow cytometry was used to observe the expression of enhanced green fluorescent protein (EGFP) in the control group. The ratio of EGFP fluorescent cells was measured and the transfection rate was calculated to evaluate the transfection effect. Results under fluorescence microscope, a large amount of EGFP expression was observed in ultrasound microbubble liposome group, which was significantly higher than that in other groups. The gene transfection efficiency in the control group, microbubble group, ultrasonic microbubble group, liposome group and ultrasound microbubble liposome group were 0.18% 卤0.09% 卤0.25% 卤0.11% 卤1.14% 卤0.13 + 2.96% 卤0.16% 卤0.12% 卤7.16% 卤0.17, respectively. The gene transfection rate in the ultrasound microbubble liposome group was higher than that in the other groups (P 0.05). Conclusion UTMD can enhance the transfection effect of liposome-mediated plasmid pSilencer3.1-SATB1 on DU145 cells and improve the transfection efficiency, which provides a new way for the treatment of prostate cancer.
【作者单位】：河南大学淮河医院;
【分类号】：R737.25

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