TRAPPC9基因对奶牛金葡菌乳房炎抗性性状的遗传效应

发布时间：2018-04-20 06:40

本文选题：奶牛 + 金黄色葡萄球菌　；参考：《畜牧兽医学报》2016年02期

【摘要】：旨在分析奶牛金黄色葡萄球菌(金葡菌)抗性候选基因TRAPPC9的遗传效应。本研究利用中国北方地区6个牛场517头具有完整DHI、年龄及胎次记录的中国荷斯坦牛的生产数据,使用Multiplex SnaPshot微测序技术分析TRAPPC9基因4个SNPs的基因型,利用特异性PCR技术检测牛只感染金葡菌情况,同时测定血清中TNF-α、IFN-γ、IL-17、NF-κB等细胞因子的含量。在此基础上,利用GLM模型最小二乘法分析TRAPPC9基因4个SNPs对奶牛金葡菌乳房炎抗性的遗传效应。结果表明:IL-17、TNF-α和IFN-γ可作为有效的乳房炎参考指标。对于金葡菌阴性牛,TRAPPC9基因SNP4(C2477531T)对SCS有极显著影响(P0.01);对于金葡菌阳性牛,该SNP对IL-17有极显著效应(P0.01)。TRAPPC9基因可作为中国荷斯坦牛金葡菌乳房炎抗性分子标记之一。
[Abstract]:The aim of this study was to analyze the genetic effects of TRAPPC9, a candidate gene for staphylococcus aureus resistance in dairy cattle. In this study, Multiplex SnaPshot microsequencing technique was used to analyze 4 SNPs genotypes of TRAPPC9 gene based on the production data of 517 Chinese Holstein cattle with complete date, age and birth order recorded from 6 cattle farms in northern China. The specific PCR technique was used to detect the infection of Staphylococcus aureus in cattle, and the levels of cytokines such as TNF- 伪, IFN- 纬, IL-17, NF- 魏 B in serum were determined. On this basis, the genetic effects of 4 SNPs of TRAPPC9 gene on mastitis resistance of staphylococcus aureus in dairy cattle were analyzed by least square method of GLM model. The results showed that TNF- 伪 and IFN- 纬 could be used as effective markers for mastitis. For Staphylococcus aureus negative bovine trapPC9 gene (SNP4C2477531T), it had a significant effect on SCS, and for Staphylococcus aureus positive cattle, the SNP gene had a significant effect on IL-17. TRAPPC9 gene could be used as one of the molecular markers of resistance to mastitis of Staphylococcus aureus in China.
【作者单位】：中国农业大学动物科技学院;西北农林科技大学食品学院;
【基金】：国家自然科学基金(31272420) 农业部奶业体系项目(CARS-37-04B) 十二五国家科技支撑项目(2011BAD28B02) 教育部基本科研项目(2011JS006) 长江学者与创新团队发展计划(IRT1191)
【分类号】：S858.23

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