金柑开花关键基因FcFT1的克隆及表达分析

发布时间：2018-04-21 01:24

  本文选题：金柑 + 克隆　； 参考：《分子植物育种》2016年03期

【摘要】：本研究以融安金柑为试材,采用同源克隆技术获得成花素基因Fc FT1全长序列,并对该基因的生物信息学和遗传进化关系进行了分析。利用q RT-PCR技术,分析了Fc FT1在花发育期间不同花器官、不同组织,以及日周期中Fc FT1在花、茎、叶的表达情况。结果表明:Fc FT1基因完整开放阅读框为531 bp,编码177个氨基酸。Fc FT1编码的蛋白具有单一而非常保守的PEBP结构域;蛋白质结构比较不稳定,为亲水蛋白。系统进化树表明Fc FT1与甜橙、温州蜜柑和枳壳中的FT同源基因亲缘关系最近。q RT-PCR结果显示,在花发育期,花药中Fc FT1表达量最高;日周期变化中,Fc FT1在花、茎、叶中的平均表达量差距不显著,表达较稳定。本研究结果为进一步揭示FT基因在金柑开花调控中的功能奠定了基础。
[Abstract]:In this study, the full-length sequence of FC FT1 was obtained by using homologous cloning technique, and the bioinformatics and genetic evolution of the gene were analyzed. The expression of FC FT1 in flower, stem and leaf during flower development was analyzed by Q RT-PCR technique in different flower organs, tissues and daily cycle. The results showed that the complete open reading frame of the 1: FC FT1 gene was 531 BP, encoding 177 amino acids. FC FT1 encoded a single and very conserved PEBP domain, and the protein structure was unstable and hydrophilic. The phylogenetic tree showed that FC FT1 was closely related to FT homologous genes in orange, bergamot and Fructus Aurantii. The results showed that FC FT1 expression was the highest in anther at flowering stage, and FC FT1 was in flower and stem during daily cycle change. The difference of average expression in leaves was not significant and the expression was stable. The results of this study laid a foundation for further revealing the function of FT gene in the regulation of flowering.
【作者单位】： 广西大学农学院亚热带农业生物资源保护与利用国家重点实验室;
【基金】：国家自然科学基金项目(31460508) 国家现代农业产业技术体系广西柑橘创新团队首席专家项目(nycytxgxcxtd-02-06)共同资助
【分类号】：S666
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本文编号：1780322