草鱼成纤维细胞gcngr1基因的表达分析

发布时间：2018-04-21 03:41

本文选题：草鱼 + ngr　；参考：《南方水产科学》2017年05期

【摘要】：Ngr1(nogo-66 receptor)是在哺乳类上发现的一种神经元受体,可调节轴突的可塑性并抑制损伤中枢神经系统(CNS)的再生,最新研究还发现它是哺乳动物呼肠孤病毒(mammalian reovirus)的神经受体。草鱼呼肠孤病毒(grass carp reovirus,GCRV)可引发草鱼(Ctenopharyngodon idellus)出血病导致高死亡率,开展GCRV受体相关研究可有助于了解病毒的致病机制。该研究在草鱼吻端成纤维细胞(PSF)中克隆到草鱼ngr1 c DNA序列(下文简称为gcngr1),发现其与哺乳动物呼肠孤病毒神经受体基因Ngr1有相似的结构序列;采用q RT-PCR方法检测该基因在PSF细胞的表达情况,结果显示受草鱼呼肠孤病毒(GCRV-GD108株)感染后gcngr1 m RNA的表达量显著上升,与病毒的增殖趋势基本一致;病毒经病毒抗体孵育后再感染PSF细胞,细胞中病毒的增殖水平下降,gcngr1m RNA的表达量也显著下降。该研究结果提示gcngr1与病毒的感染相关,为进一步分析gcngr1是否为GCRV神经元受体提供依据。
[Abstract]:Ngr1(nogo-66 receptor, a neuronal receptor found in mammals, regulates the plasticity of axons and inhibits the regeneration of injured central nervous system (CNS). It is also found to be the receptor of mammalian reovirus mammreovirus. Grass grass reovirus (grass grass reovirus) can cause high mortality caused by grass carp grass carp (Ctenopharyngodon idellus) haemorrhage. The study of GCRV receptor may be helpful to understand the pathogenesis of the virus. In this study, the ngr1 c DNA sequence was cloned from the rostral fibroblast of grass carp (gcngr1). It was found that the sequence was similar to that of mammalian reovirus neuroreceptor gene Ngr1. Q RT-PCR assay was used to detect the expression of the gene in PSF cells. The results showed that the expression of gcngr1 m RNA increased significantly after infection with grass carp reovirus GCRV-GD108 strain, which was basically consistent with the proliferation trend of the virus. After the virus was incubated with the virus antibody, the virus was then infected with PSF cells, and the level of virus proliferation was decreased. The expression of gcngr1m RNA in the cells was also significantly decreased. The results suggest that gcngr1 is associated with viral infection and provide a basis for further analysis of whether gcngr1 is a GCRV neuron receptor.
【作者单位】：中国水产科学研究院珠江水产研究所农业部热带亚热带水产资源利用与养殖重点实验室;上海海洋大学水产与生命学院;
【基金】：中国水产科学研究院中央级公益性科研院所基本科研业务费专项资金资助(2016HY-ZC0602) 广东省级鱼病防治专项资金项目“草鱼病毒特异性疫苗研究与开发”
【分类号】：S943

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