茶树单萜合成酶CsTPS基因的克隆及表达分析

发布时间：2018-04-21 05:04

本文选题：茶树 + 单萜合成酶　；参考：《西北植物学报》2017年08期

【摘要】：该研究在茶树转录组测序的基础上,以‘铁观音’茶树品种的芽叶为供试材料,采用RT-PCR技术,克隆了茶树的1条单萜合成酶(TPS)基因全长cDNA序列,命名为CsTPS(GenBank登录号为KY829105)。CsTPS基因全长2 077bp,其开放阅读框(ORF)为1 752bp,编码583个氨基酸。CsTPS基因编码蛋白含有单萜合成酶蛋白特有的2个天冬氨酸富集基序及RRx8W、RxR保守基序,N端具有一段叶绿体转运肽。同源比对分析显示,CsTPS氨基酸序列与多种植物的单萜合成酶序列相似性较高,与黄胡萝卜α-松油醇合成酶、白|柠檬烯合成酶、蓖麻单萜合成酶相似性分别为74%、71%和66%。系统进化树分析表明,CsTPS属于TPSb亚家族类型。荧光定量PCR结果显示,在白茶萎凋、乌龙茶做青、红茶发酵等加工过程中,CsTPS基因的表达量均有上调,推测CsTPS基因的表达与茶叶加工过程中茶叶香气形成密切相关。
[Abstract]:Based on the sequencing of tea transcriptome, the full-length cDNA sequence of a monoterpene synthase (TPS) gene was cloned from the bud leaves of tea variety 'Tieguanyin' by using RT-PCR technique. CsTPS(GenBank accession number is 2077bp. its open reading frame (ORF) is 1752 BP, encoding 583 amino acids. CsTPS gene encoding protein contains two aspartic acid rich motifs unique to monoterpene synthase and N terminal of RRx8WN RxR conserved motif. There is a chloroplast transporter peptide. Homologous alignment analysis showed that the amino acid sequence of CsTPS was similar to that of monoterpene synthase of many plants, and the similarity with 伪 -terpineol synthase, limonene synthase and castor monoterpene synthase was 74% and 66%, respectively. Phylogenetic tree analysis showed that CsTPS belonged to TPSb subfamily. The results of fluorescence quantitative PCR showed that the expression of CsTPS gene was up-regulated during the processing of white tea withering, oolong tea green and black tea fermentation. It was inferred that the expression of CsTPS gene was closely related to the formation of tea aroma during tea processing.
【作者单位】：福建农林大学园艺学院/茶学福建省高校重点实验室;
【基金】：福建省“2011协同创新中心”中国乌龙茶产业协同创新中心专项(闽教科[2015]75号) 国家现代农业(茶叶)产业技术体系建设专项(CARS-19) 福建省自然科学基金(2017J0107)
【分类号】：Q943.2;S571.1

【相似文献】