羽叶三七的转录组测序与三萜皂苷生物合成的关键酶基因的识别

发布时间：2018-04-21 08:52

  本文选题：羽叶三七 + 转录组测序　； 参考：《基因组学与应用生物学》2017年06期

【摘要】：羽叶三七是五加科人参属的名贵药材,三萜皂苷为羽叶三七最主要的活性成分。为了探索羽叶三七根茎中皂苷物质生物合成的分子基础,采用Illumina Hi Seq 2000高通量测序获得羽叶三七根茎的转录组数据;使用Trinity和TGICL软件实现Uni Gene的de novo拼接;基于BLAST完成Uni Gene的蛋白功能注释、KOG功能注释、GO分类和KEGG代谢通路分析。最终通过de novo拼接注释得到Uni Gene 62 240个。研究发现,羽叶三七根茎部表达的26个Uni Gene与三萜碳环骨架合成相关;三萜合成通路中的关键酶FPS、SS、SE等,分别有11 114个Uni Gene。该研究发现的三萜皂苷合成相关候选基因对于阐明羽叶三七三萜皂苷合成方式研究提供了理论基础。
[Abstract]:Panax notoginseng is a valuable medicinal material of Panax ginseng, and triterpenoid saponins are the main active components of Panax notoginseng. In order to explore the molecular basis of saponin biosynthesis in the rhizome of Panax notoginseng, Illumina Hi Seq 2000 high-throughput sequencing was used to obtain the transcriptional data of the rhizome of Panax notoginseng, and the de novo splicing of Uni Gene was realized by Trinity and TGICL software. BLAST was used to complete the functional annotation of Uni Gene: go classification and KEGG metabolic pathway analysis. Finally, Uni Gene 62,240 were obtained by de novo splicing annotation. It was found that 26 Uni Gene expressed in rhizome of Panax notoginseng were related to the synthesis of triterpenoid carbon cytoskeleton, and 11 114 Uni genes were found to be the key enzymes in triterpene synthesis pathway. The candidate genes related to triterpenoid saponins biosynthesis provided a theoretical basis for elucidating the synthesis of triterpenoid saponins in leaves.
【作者单位】： 武汉轻工大学生物与制药工程学院;
【基金】：国家自然科学基金(No.81274023)资助
【分类号】：Q943.2;S567.5
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本文编号：1781741