牦牛KLF5、KLF6、KLF7基因的克隆表达及其与肌内脂肪含量的相关性分析

发布时间：2018-04-22 04:02

本文选题：牦牛 + KLFs　；参考：《畜牧兽医学报》2017年03期

【摘要】：本研究旨在克隆牦牛Krüppel样因子(KLF5、KLF6、KLF7)的CDS区序列,鉴定其在牦牛不同组织中的表达水平,并分析其表达水平与牦牛肌内脂肪(IMF)的相关性,从而为研究KLFs在牦牛脂质代谢中的功能提供试验依据。选取4~6周岁的健康麦洼牦牛公牛7头,清晨空腹屠宰后,迅速采集脾、肺、肾、皮下脂肪和背最长肌组织样品,提取组织总RNA,利用RT-PCR法克隆牦牛KLF5、KLF6、KLF7基因序列,进行生物信息学分析,利用实时定量PCR法检测其mRNA的表达水平,并使用皮尔逊相关系数进行KLF5、KLF6、KLF7基因的表达量与IMF含量的相关性分析。结果表明,KLF5、KLF6、KLF7基因CDS全长分别为1 086、855和909bp,分别编码361、284和302个氨基酸残基,且均与牛具有最近的亲缘关系,其次为山羊、绵羊、猪,而与鸡的亲缘关系较远。KLF5、KLF6、KLF7均在肺中具有较高的表达水平,而在背最长肌中的表达量较低,且与牦牛IMF含量均没有显著相关性。但KLF5的表达水平与KLF6、KLF7均具有显著的正相关性。这些结果为研究KLF5、KLF6、KLF7的功能提供了基础数据,也为揭示牦牛脂质代谢的调控机制提供了试验参考。
[Abstract]:The aim of this study was to clone the CDS region of yak Kr 眉 ppel like factor KLF5, KLF6 and KLF7, to identify its expression level in different tissues of yak, and to analyze the correlation between the expression level and the intramuscular fat of yak. So as to study the function of KLFs in yak lipid metabolism. Seven healthy Mai WA yak bulls aged 4 to 6 years old were selected. After slaughtering on an empty stomach in the morning, samples of spleen, lung, kidney, subcutaneous fat and longissimus dorsi muscle tissue were collected, and the total RNAs were extracted. The gene sequence of KLF5UKLF6F6 KLF7 gene was cloned by RT-PCR. The expression level of mRNA was detected by real-time quantitative PCR and Pearson correlation coefficient was used to analyze the correlation between the expression of KLF5, KLF6 and KLF7 gene and the content of IMF. The results showed that the CDS length of KLF6 gene was 1 086855 and 909bprespectively, encoding 361284 and 302 amino acid residues, respectively, and had the closest relationship with cattle, followed by goat, sheep and pig. The relationship between KLF5, KLF6 and KLF7 was higher in the lung, but lower in the longissimus dorsi muscle, and there was no significant correlation between the expression of KLF6 and KLF7 in the longissimus dorsi muscle and the content of IMF in yak. But the expression level of KLF5 was positively correlated with KLF6 and KLF7. These results provide basic data for studying the function of KLF5, KLF6 and KLF7, and provide experimental reference for revealing the regulation mechanism of lipid metabolism in yak.
【作者单位】：青藏高原动物遗传资源保护与利用四川省重点实验室;动物遗传育种学国家民委-教育部重点实验室;
【基金】：四川省青年科技创新团队项目(2015TD0025)
【分类号】：S823.85

【参考文献】