菊芋果聚糖:果聚糖1-果糖基转移酶1-FFT基因的克隆及表达分析

发布时间：2018-04-22 13:14

本文选题：菊芋 + -FFT基因　；参考：《分子植物育种》2016年02期

【摘要】：果聚糖作为一种重要的渗透调节物质,与植物碳素分配和抗逆性的密切相关。为明确果聚糖在植物生长发育过程中的作用,本研究以菊芋为材料,克隆出果聚糖:果聚糖1-果糖基转移酶1-FFT基因,其CDS长2 025 bp,编码641个氨基酸,该蛋白的分子量(Mw)为73.906 k D,蛋白的等电点(p I)为10.01。进化树分析显示菊芋中1-FFT基因与维氏菊的1-FFT基因进化距离最近,具有较高的保守性。荧光定量检测结果显示,1-FFT基因在菊芋的叶、根、花、茎和块茎中均有表达,其中在块茎中表达量最高。本研究为解析果聚糖合成酶的分子调控机理及功能鉴定提供依据。
[Abstract]:As an important osmotic regulator, fructan is closely related to plant carbon distribution and resistance to stress. In order to clarify the role of fructan in plant growth and development, the fructan 1 fructosyltransferase (1-FFT) gene was cloned from Jerusalem artichoke. Its CDS length was 2 025 BP and encoded 641 amino acids. The molecular weight of the protein is 73.906 KD and the isoelectric point of the protein is 10.01. Phylogenetic tree analysis showed that the evolution distance between 1-FFT gene and 1-FFT gene of Chrysanthemum Vickers was close, and it was highly conserved. The results of fluorescence quantitative analysis showed that the 1-FFT gene was expressed in the leaves, roots, flowers, stems and tubers of Jerusalem artichoke, and the highest expression was found in the tubers. This study provides the basis for elucidating the molecular regulation mechanism and functional identification of fructan synthase.
【作者单位】：青海大学农林科学院菊芋研发中心青海省蔬菜遗传与生理重点实验室;中国科学院西北高原生物研究所;
【基金】：国家自然科学基金项目(31460523) 青海省科技厅应用基础研究项目(2013-Z-718)共同资助
【分类号】：S632.9

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