一个水稻温敏黄化突变体的表型分析和基因定位

发布时间：2018-04-22 20:30

本文选题：水稻 + 温度敏感型　；参考：《作物学报》2017年10期

【摘要】：对水稻叶色进行表型分析与基因定位,可为图位克隆该类基因和研究水稻光合系统功能奠定基础。利用甲基磺酸乙酯(ethyl methanesulfonate,EMS)诱变的方法,从籼稻品种"南京11"(简称NJ11)中获得温敏黄化突变体dy1;与野生型相比,dy1在自然环境下,从苗期至成熟期始终表现叶片黄化,透射电镜显示dy1类囊体结构异常;同时株高、分蘖数、结实率等农艺性状也表现出极显著的差异;实验室光照培养箱条件下,dy1苗期在20℃下表现白化,在25℃表现黄化,在30℃下为浅绿的表型;遗传分析表明水稻温敏黄化突变体dy1的表型是由1对隐性基因控制;将突变体与粳稻广亲和品"02428"杂交,构建F2群体,从中选出隐性极端个体,通过基因定位,将控制黄化的基因限定在第1染色体长臂上标记Y-4和Y-35之间的115 kb的区间内,含有16个开放阅读框(ORF),测序发现,dy1中编码尿嘧啶核苷酸激酶的基因LOC_Os01g73450的第4个内含子与第5个外显子交界处存在单碱基替换,拟作为候选基因;且叶绿体合成相关基因表达量显著降低,猜测该基因可能参与了叶绿素合成途径。
[Abstract]:The phenotypic analysis and gene mapping of rice leaf color can lay a foundation for the mapping cloning of these genes and the study of the function of rice photosynthetic system. The thermo-sensitive yellowing mutant dy1 was obtained from the indica rice variety "Nanjing 11" (NJ11) by the method of mutagenesis of ethyl methanesulfonate EMS.Compared with the wild type, it showed leaf yellowing from seedling to maturity in natural environment. Transmission electron microscopy showed that dy1 thylakoid structure was abnormal, at the same time, plant height, tiller number, seed setting rate and other agronomic traits also showed significant differences. Genetic analysis showed that the phenotype of rice thermo-sensitive yellow mutant dy1 was controlled by a pair of recessive genes, and F _ 2 population was constructed by crossing the mutant with japonica rice wide compatibility line "02428", from which the recessive extreme individuals were selected. By gene mapping, the genes controlling yellowing were limited to 115kb between Y-4 and Y-35 labeled on the long arm of chromosome 1. It was found that the fourth intron of LOC_Os01g73450, which encodes uropyrimidine nucleotide kinase, had a single base substitution at the junction of the fifth exon and the fourth intron of the nucleotide kinase encoding uropyrimidine nucleotide kinase, and it was suggested that it could be used as a candidate gene. The expression of chloroplast biosynthesis related gene was significantly decreased, suggesting that the gene might be involved in chlorophyll synthesis pathway.
【作者单位】：南京农业大学作物遗传与种质创新国家重点实验室/农业部长江中下游粳稻生物学与遗传育种重点实验室/江苏省植物基因工程技术研究中心;中国农业科学院;
【基金】：国家重点研发计划项目(2016YFD0100101-08) 江苏省科技支撑计划项目(BE2015363) 江苏省农业科技自主创新项目[CX(16)1029] 安徽省科技重大专项(16030701068)资助~~
【分类号】：S511

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