家养双峰驼SRY基因的序列分析与拷贝数研究

发布时间：2018-04-22 22:18

本文选题：家养双峰驼 + SRY基因　；参考：《西北农业学报》2017年11期

【摘要】：为探究中国家养双峰驼SRY基因的序列以及群体间拷贝数变异,对7个中国家养双峰驼群体共94个个体的SRY基因部分序列进行测序,分析SRY基因序列与其他物种的相似性;并且以CYP2A基因为内参基因,运用实时荧光定量PCR技术检测SRY基因的拷贝数。结果表明,中国家养双峰驼与人、马、猪、羊及普通牛的SRY基因相似度分别为67.7%~68.1%、68.5%~69.4%、73.8%~74.4%、73.8%~74.8%、74.1%~74.8%。在家养双峰驼SRY基因的多个拷贝中,除1个拷贝外,其余拷贝的HMG序列与羊驼、人、马、猪、绵羊及普通牛的HMG序列相比,相似度分别为97.3%、84.2%、85.6%、86.3%、87.0%、85.6%。SRY基因在中国家养双峰驼Y染色体上以多拷贝形式存在,变幅为1~9个拷贝。表明中国家养双峰驼SRY基因的HMG序列是高度保守的,而且该基因属于多拷贝基因。
[Abstract]:In order to investigate the sequence of SRY gene and the variation of copy number among populations, the partial sequence of SRY gene of 94 individuals in 7 Chinese bactrian camel populations was sequenced, and the similarity between SRY gene sequence and other species was analyzed. The copy number of SRY gene was detected by real-time fluorescence quantitative PCR using CYP2A gene as internal reference gene. The results showed that the similarity of SRY gene between Chinese bactrian camel and human, horse, pig, sheep and common cattle was 67.7% and 68.5%, respectively. Among the multiple copies of the SRY gene of Bactrian Camels, except one copy, the other copies of the HMG sequence were compared with the HMG sequences of alpaca, human, horse, pig, sheep and common cattle. The similarity between 97.3 and 84.2and 86.3and 86.3and 87.0and 85.6respectively. The SRY gene exists in the form of multiple copies on the Y chromosome of Chinese bactrian camel, with a variation of 1 ~ 9 copies. The results showed that the HMG sequence of SRY gene was highly conserved and the gene belonged to multi-copy gene.
【作者单位】：西北农林科技大学动物科技学院;
【基金】：国家自然科学基金(31172178)~~
【分类号】：S824

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