铁过载小鼠心肌组织JWA基因mRNA的表达及其与氧化应激损伤的关系研究

发布时间：2018-04-23 03:40

本文选题：铁过载 + JWA　；参考：《现代预防医学》2017年08期

【摘要】：目的探讨铁过载对雄性小鼠心肌组织JWA基因m RNA表达的影响及其与氧化应激损伤的相关性。方法 40只雄性小鼠按体重随机分成4组(对照组和3个铁过载组),各组隔天腹腔注射不同剂量(0、50、100、150 mg/kg·wb)的右旋糖苷铁,持续注射4周后,眼球取血测定血清铁;处死小鼠,取肝脏组织测肝组织铁,取心肌组织,RT-PCR技术测心肌组织JWA基因mRNA表达量,并测心肌组织丙二醛(MDA)、过氧化氢酶(CAT)、过氧化氢(H_2O_2)、羟自由基和谷胱甘肽过氧化物酶(GSH-PX)含量。结果与对照组相比,3个过载组血清铁及肝组织铁含量均显著升高(P值均0.05);100 mg/kg·wb剂量组、150 mg/kg·wb剂量组铁过载小鼠心肌组织的JWA基因mRNA表达水平显著升高,差异均有统计学意义(P0.05)。心肌组织JWA基因mRNA表达量与其CAT活力、过氧化氢含量、GSH-PX水平和羟自由基活力呈负相关关系,而与MDA含量呈正相关关系(P0.05)。氧化应激水平显示,与对照组相比,3个铁过载组心肌匀浆MDA含量均升高(P0.05);而CAT活力、H_2O_2含量、羟自由基活力、GSH-PX均降低(P0.05)。结论铁过载导致小鼠心肌组织JWA基因mRNA高表达,并与心肌组织氧化应激损伤密切相关。
[Abstract]:Objective to investigate the effect of iron overload on the expression of JWA gene m RNA in myocardial tissue of male mice and its correlation with oxidative stress injury. Methods Forty male mice were randomly divided into 4 groups according to their body weight (control group and three iron overload groups). Each group was injected intraperitoneally with different doses of ferric dextran 50100150 mg/kg wb every other day. After 4 weeks of continuous injection, blood samples were taken from the eyeball to determine serum iron, and the mice were killed. The levels of JWA gene mRNA, malondialdehyde (MDA), catalase (catalase), H _ 2O _ 2 (H _ 2O _ 2), hydroxyl radical and glutathione peroxidase (GSH-PX) in myocardial tissue were measured by reverse transcription-polymerase chain reaction (RT-PCR). Results compared with the control group, the levels of serum iron and hepatic iron in the three overload groups were significantly higher than those in the control group (P < 0.05). The expression of JWA gene mRNA in the myocardium of the iron-overload mice in the dose group of 150 mg/kg wb was significantly higher than that in the control group, and the difference was statistically significant (P 0.05). The expression of JWA gene mRNA was negatively correlated with the activity of CAT, the level of GSH-PX and the activity of hydroxyl radical, but positively correlated with the content of MDA. The level of oxidative stress showed that compared with the control group, the content of MDA in myocardial homogenate of the three iron overload groups was increased, while the activity of CAT and the activity of hydroxyl radical GSH-PX were all decreased. Conclusion the high expression of JWA gene mRNA in myocardial tissue induced by iron overload is closely related to oxidative stress injury in myocardial tissue.
【作者单位】：南昌大学研究生院医学部;南昌大学公共卫生学院;
【基金】：江西省教育厅科技项目(GJJ14091)
【分类号】：R54

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