宫颈癌细胞系中EMT相关基因的表达及其意义

发布时间：2018-04-23 11:30

本文选题：宫颈癌 + 上皮-间质转化(EMT)　；参考：《西安交通大学学报(医学版)》2017年02期

【摘要】：目的上皮-间质转化(EMT)是上皮来源的恶性肿瘤侵袭转移的重要机制之一,其特征为上皮细胞标志物表达下调(E-cadherin等)而间质细胞标志物(vimentin等)表达上调。本研究旨在探讨人宫颈癌细胞系EMT相关标志物的表达情况,以确定宫颈癌细胞是否发生EMT,并为确定相关的细胞研究模型奠定基础。方法采用逆转录PCR方法检测4株人宫颈癌细胞系(SiHa、HeLa、RJC-1、CS1213)中E-cadherin、vimentin、Snail和Slug mRNA的表达;Western blotting检测E-cadherin和vimentin的蛋白表达;免疫细胞化学方法检测Snail和Slug的蛋白表达。结果vimentin和Snail mRNA在4种细胞中均有表达,E-cadherin和Slug mRNA在SiHa、HeLa和RJC-1细胞表达。E-cadherin蛋白在SiHa和RJC-1细胞表达,vimentin蛋白在HeLa和CS1213细胞表达。Snail蛋白在4个细胞株都有表达,Slug蛋白在SiHa、HeLa和RJC-1细胞表达。结论 HeLa和CS1213表现出间质细胞表型,SiHa和RJC-1则表现为上皮细胞表型。
[Abstract]:Objective EMTs are one of the important mechanisms of invasion and metastasis of epithelial malignant tumors, characterized by down-regulated expression of epithelial cell markers (E-cadherin, etc.) and up-regulated expression of mesenchymal markers (vimentin). The purpose of this study was to investigate the expression of EMT related markers in human cervical cancer cell lines, and to determine whether or not EMTs occur in cervical cancer cells, and to lay a foundation for determining the relevant cell research models. Methods the expression of E-cadherin vimentinon Snail and Slug mRNA in four human cervical cancer cell lines, SiHaHN HeLaRJC-1rJC-1 and CS1213, was detected by reverse transcription PCR. The expression of E-cadherin and vimentin was detected by Western blotting, and the protein expression of Snail and Slug was detected by immunocytochemistry. Results the expression of E-cadherin and Slug mRNA in SiHa-HeLa and RJC-1 cells was found in both vimentin and Snail mRNA. E-cadherin protein was expressed in SiHa and RJC-1 cells. Snail protein was expressed in HeLa and CS1213 cells. Slug protein was expressed in SiHaHeLa and RJC-1 cells. Conclusion HeLa and CS1213 showed interstitial cell phenotype SIHA and RJC-1 as epithelial cell phenotypes.
【作者单位】：西安交通大学第一附属医院病理科;西安交通大学第一附属医院检验科;西安交通大学第一附属医院转化医学中心;
【基金】：国家自然科学基金资助项目(No.30872928)~~
【分类号】：R737.33

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