大口黑鲈肌球蛋白重链基因SNPs的筛选及与生长性状的关联

发布时间：2018-04-23 22:02

本文选题：大口黑鲈 + 肌球蛋白重链基因　；参考：《水产学报》2018年03期

【摘要】：肌球蛋白是肌肉细胞的重要组成成分,影响肌纤维的组成和肌肉生长。为了研究肌球蛋白重链(MYH)基因多态性与大口黑鲈生长性状的相关性,本研究采用PCR技术扩增得到编码区序列全长为9759 bp的大口黑鲈MYH基因,该基因包含37个外显子和36个内含子,编码1940个氨基酸。采用直接测序法在MYH基因上筛选到8个单核苷酸多态性标记(SNP)位点(A-305G、G-558C、A-2784C、A-2816G、T-4765A、C-6206T、C-6811T和G-6935T),有4个位于外显子上,其中2个属于同义突变。用SNa Pshot方法对从同批繁殖、同塘养殖的大口黑鲈"优鲈1号"群体中随机选取的430尾个体中各位点的基因型进行检测。结果显示,内含子上的A-2784C和A-2816G位点完全连锁,所有位点在大口黑鲈"优鲈1号"群体中的平均有效等位基因数、平均观测杂合度和平均期望杂合度分别为1.635、0.406和0.373,仅C-6206T、C-6811T和T-4765A位点的基因型频率分布符合Hardy-Weinberg定律。采用一般线性模型分析各位点与大口黑鲈生长性状之间的相关性,研究发现,C-6811T位点CC基因型个体的体质量和全长显著大于TT基因型,CC基因型个体的体高和尾柄长显著大于CT和TT基因型,其余位点不同基因型个体间的生长性状均不存在显著差异。C-6811T位点与生长性状显著相关,可作为大口黑鲈分子标记辅助育种的候选标记。
[Abstract]:Myosin is an important component of muscle cells, which affects muscle fiber composition and muscle growth. In order to study the association between myosin heavy chain myosin (Myh) gene polymorphism and growth traits of large mouth black bass, the MYH gene with coding region of 9759 BP was amplified by PCR technique. The gene contains 37 exons and 36 introns, encoding 1940 amino acids. Eight single nucleotide polymorphisms (SNPs) loci (A-305GN G-558CU A-2816GN T-4765TNC-6811T and G-6935T) were screened on MYH gene by direct sequencing. Four of them were located on exons, 2 of which were synonymous mutations. SNa Pshot method was used to detect the genotypes of each of the 430individuals randomly selected from the population of "you Perch 1", which was bred in the same batch and cultured in Tongtang. The results showed that the A-2784C and A-2816G loci on the intron were completely linked, and the average effective allele number of all the loci in the population of "you Perch 1" was found. The average observed heterozygosity and average expected heterozygosity were 1.6350.406 and 0.373, respectively. Only the genotype frequencies of C-6206TX C-6811T and T-4765A loci were in accordance with Hardy-Weinberg 's law. A general linear model was used to analyze the correlation between the growth traits of Lateolchus japonicus and each point. It was found that the body weight and total length of CC genotype individuals at C-6811T locus were significantly higher than those of CT and TT genotypes, and the body height and tail stalk length of CC genotype individuals with TT genotype were significantly higher than those of CT and TT genotypes. There was no significant difference in growth traits among individuals with different genotypes in other loci. C-6811T loci were significantly correlated with growth traits, and could be used as candidate markers for molecular marker-assisted breeding of large mouth black bass.
【作者单位】：中国水产科学研究院珠江水产研究所农业部热带亚热带水产资源利用与养殖重点实验室;上海海洋大学水产与生命学院;内江师范学院;
【基金】：渔港建设和渔业产业发展专项(A201601A12) 广东省科技计划项目(2016A020210020,2015A020209035) 浙江省农业(水产)新品种选育重大科技专项(2016C02055-3)~~
【分类号】：S917.4

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