连续睡眠剥夺对大鼠肝生物钟基因及肝损伤标志物表达水平的影响

发布时间：2018-04-24 04:34

本文选题：连续睡眠剥夺 + 生物钟基因　；参考：《军事医学》2017年03期

【摘要】：目的探讨36 h连续睡眠剥夺(SD)对大鼠肝、肾生物钟基因表达水平及大鼠尿液中肝损伤标志物总胆汁酸(TBA)含量的影响。方法将12只大鼠随机分为正常对照组、睡眠剥夺组(SD组)。SD组大鼠使用睡眠剥夺仪进行睡眠剥夺,连续36 h后,暴露腹腔取肝、肾,用RT-PCR方法检测大鼠肝、肾组织中生物钟基因转录表达水平的变化情况,并对生物钟基因的蛋白表达产物进行免疫印迹分析;暴露盆腔取尿液,用ELISA试剂盒检测大鼠尿液中肝损伤标志物TBA含量的变化情况。结果与对照组相比,SD组大鼠肝生物钟基因clock,bmal1的mRNA表达水平明显下调(P0.05),肾同种生物钟基因表达水平在睡眠剥夺前后无明显变化。肝生物钟基因的蛋白表达水平与转录水平一致。同时,SD组大鼠与对照组大鼠相比尿液中TBA含量明显上调(P0.001)。结论 36 h连续睡眠剥夺可导致大鼠肝生物钟基因表达和胆固醇代谢紊乱,TBA可作为昼夜节律紊乱致肝损伤效应的无创性生物标志物。
[Abstract]:Objective to investigate the effects of 36 h continuous sleep deprivation (SDN) on the expression of liver and kidney clock gene and the content of total bile acid (TBA) in urine of rats. Methods Twelve rats were randomly divided into normal control group, sleep deprivation group (SD group) and SD group (SD group). After 36 hours of sleep deprivation, liver and kidney were taken from abdominal cavity and liver was detected by RT-PCR method. Changes in the transcription and expression level of the biological clock gene in renal tissue, and Western blot analysis of the protein expression products of the biological clock gene. ELISA kit was used to detect the changes of liver injury marker TBA in urine of rats. Results compared with the control group, the expression of liver clock gene clock bmal1 mRNA in SD group was significantly down-regulated (P 0.05), while the expression level of kidney allogeneic clock gene had no significant change before and after sleep deprivation. The protein expression level of the liver clock gene is consistent with the transcriptional level. At the same time, the content of TBA in urine of SD group was significantly up-regulated than that of control group (P 0.001). Conclusion 36 h continuous sleep deprivation can lead to liver clock gene expression and cholesterol metabolism disorder TBA can be used as a non-invasive biomarker of liver injury induced by circadian rhythm disorder.
【作者单位】：兰州大学基础医学院病理学研究室;军事医学科学院基础医学研究所应激医学研究室;
【基金】：军队“十二五”重大资助项目(AWS14J011) 北京市自然科学基金青年基金资助项目(7164287) 细胞应激生物学国家重点实验室(厦门大学)开放基金资助项目(SKLCSB2016KF010)
【分类号】：R575

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