姜黄素对朱砂叶螨几丁质酶基因表达的影响

发布时间：2018-04-24 09:33

本文选题：姜黄素 + 朱砂叶螨　；参考：《农药学学报》2016年02期

【摘要】：为明确植物源活性物质姜黄素对朱砂叶螨Tetranychus cinnabarinus的杀螨作用机理,克隆并分析了6条朱砂叶螨几丁质酶基因(Tc CHTs)的c DNA序列,利用RT-q PCR技术,测定了不同发育阶段的螨体内几丁质酶基因表达的情况,同时研究了姜黄素作用下几丁质酶基因表达的变化。结果表明:6条几丁质酶基因(Tc CHIT1、Tc CHIT2、Tc CHIT3、Tc CHT4、Tc CHT5和Tc CHT6)有不同的结构域,预测其蛋白质分子质量分别为60.80、69.03、104.32、59.75、45.63和49.05 k Da,等电点分别为5.44、8.65、6.23、6.07、6.12和8.27。6条基因的表达量均为幼螨和若螨时期显著高于卵和成螨时期。不同浓度姜黄素处理朱砂叶螨若螨24 h后,6条基因的表达量均有不同程度下调,其中Tc CHIT1和Tc CHT5的下调更为显著。研究结果表明,姜黄素对朱砂叶螨的杀螨作用机制之一可能是通过抑制几丁质酶的活性,进而影响几丁质的降解过程,最终因螨体壁受损而导致螨不能正常生长发育。
[Abstract]:In order to clarify the mite killing mechanism of curcumin on Tetranychus cinnabarinus, the c DNA sequences of 6 chitinase genes of Tetranychus cinnabarinus were cloned and analyzed. RT-q PCR technique was used. The chitinase gene expression in mites at different developmental stages was determined and the changes of chitinase gene expression in curcumin were studied. The results showed that Tc CHIT1, Tc CHIT2, Tc CHIT3, Tc CHT4, Tc CHT5 and Tc CHT6) had different domains, and Tc CHIT1, Tc CHIT2, Tc CHIT2, Tc CHIT3, Tc CHT4Tc CHT5 and TcCHT6) had different domains. The predicted molecular weight of its protein was 60.800.03104.32.59.75 and 49.05 K Da.The isoelectric point was 5.44.66.236.076.12 and 8.27.6 respectively. The expression levels of the genes in juvenile mites and if mites were significantly higher than those in eggs and adult mites. After 24 h treatment with curcumin, the expression of 6 genes was down-regulated, especially Tc CHIT1 and Tc CHT5. The results showed that one of the mechanisms of curcumin against Tetranychus cinnabarinus was to inhibit the activity of chitinase and then affect the degradation process of chitin, which resulted in the mites unable to grow and develop normally due to the damage of the mite's body wall.
【作者单位】：西南大学植物保护学院;
【基金】：国家自然科学基金项目(31272058和31572041)
【分类号】：S482.52

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