生物钟基因PER2对人口腔鳞癌SCC15细胞增殖、凋亡以及生物钟基因表达的影响

发布时间：2018-04-24 13:33

本文选题：癌 + 口腔　；参考：《重庆医科大学》2017年硕士论文

【摘要】：目的探讨人口腔鳞癌SCC15细胞中生物钟基因PER2的表达改变对细胞增殖、凋亡以及其它生物钟基因的影响。方法通过构建shRNA干扰人口腔鳞癌SCC15细胞中PER2基因;应用流式细胞仪检测口腔鳞癌细胞的增殖和凋亡水平的改变情况;实时荧光定量PCR检测生物钟基因CLOCK、BMAL1、PER1、PER3、DEC1、DEC2、CRY1、CRY2、TIM、CKIε、RORα、NPAS2和REV-ERBαmRNA表达。结果PER2沉默之后,SCC15癌细胞的凋亡降低,增殖加强(P0.05);SCC15细胞中生物钟基因PER3、BMAL1、DEC1、DEC2、CRY2、TIM、RORα和NPAS2 mRNA的表达水平显著降低,PER1和REV-ERBαmRNA的表达显著升高(P0.05)。结论在癌细胞中,生物钟基因PER2对生物钟基因网络中其它生物钟基因具有重要调控作用,PER2表达降低导致细胞增殖增加和凋亡水平下降。
[Abstract]:Objective to investigate the effect of PER2 expression on cell proliferation, apoptosis and other clock genes in human oral squamous cell carcinoma (SCC15) cells. Methods shRNA was constructed to interfere with the expression of PER2 gene in human oral squamous cell carcinoma (SCC15) cells, flow cytometry was used to detect the changes of cell proliferation and apoptosis in oral squamous carcinoma cells, and real-time quantitative PCR was used to detect the expression of REV-ERB 伪 -NPAS2 and REV-ERB 伪 mRNA in human oral squamous cell carcinoma cells. Results after PER2 silencing, the apoptosis of SCC15 cells was decreased, and the expression of clock gene PER3, BMAL1, DEC2, CRY2, TIMROR 伪 and NPAS2 mRNA in SCC15 cells was significantly decreased. The expression of REV-ERB 伪 and PER1 was significantly increased in SCC15 cells. Conclusion in cancer cells, PER2 plays an important role in regulating the expression of other clock genes in the biological clock gene network. The decrease of PER2 expression leads to the increase of cell proliferation and the decrease of apoptosis.
【学位授予单位】：重庆医科大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R739.8

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