龙眼DCL家族基因的启动子分析及时空表达

发布时间：2018-04-25 08:38

  本文选题：龙眼 + DCL基因　； 参考：《西北植物学报》2017年10期

【摘要】：为了解龙眼DCL基因的功能,该试验对龙眼基因组数据提取的DlDCL1、DlDCL2、DlDCL3和DlDCL4基因序列进行启动子顺式作用元件及其受miRNA调控的分析;并以龙眼胚性愈伤组织为材料,研究了DlDCLs不同基因成员在非生物胁迫和外源激素处理下的表达情况。结果显示:(1)龙眼DCL基因启动子中除了TATA和CAAT外,还具有大量的光反应元件、激素应答元件、胁迫响应元件、组织特异性调控元件及植物生长发育相关的顺式调控元件,提示龙眼DCL基因启动子转录活性可能受到光、激素信号及逆境胁迫因素的诱导。(2)对调控龙眼DCL基因的miRNA进行筛选,结果显示DlDCL1受miR162和miR1024调控,DlDCL4受miR390和miR396调控。(3)实时荧光定量PCR显示,在一定浓度范围内,外源激素GA3、ABA和ETH均能下调DlDCLs基因的表达,而高浓度ETH处理则显著上调DlDCLs的表达。(4)高浓度蔗糖(6%)处理时DlDCL2、DlDCL3和DlDCL4显著上调表达,而低浓度(0.1%)处理时DlDCL1显著上调表达;不同温度处理下,DlDCL1在34℃时显著上升,DlDCL3随着温度的提高相对表达量逐渐减低;而DlDCL2和DlDCL4表达量差异不明显;NaCl胁迫处理下,DlDCLs在1h处理时表达量下调,但在其他不同时间点则上调表达。研究表明,龙眼DCL基因在外源激素及非生物胁迫处理下,并非是简单的一对一响应,而是存在较为复杂的响应机制。
[Abstract]:In order to understand the function of longan DCL gene, the promoter cis-acting elements of DlDCL1, DlDCL2, DlDCL3 and DlDCL3 gene sequences extracted from longan genome data and their regulation by miRNA were analyzed, and the embryogenic callus of longan was used as a material. The expression of different gene members of DlDCLs under abiotic stress and exogenous hormone treatment was studied. The results showed that in addition to TATA and CAAT, the promoter of longan DCL gene also had a large number of photoresponse elements, hormone response elements, stress response elements, tissue specific regulatory elements and cis-regulatory elements related to plant growth and development. The results suggested that the transcriptional activity of the promoter of longan DCL gene might be induced by light, hormone signal and stress factors. The results showed that DlDCL1 was regulated by miR162 and miR1024. DlDCL4 was regulated by miR390 and miR396. DlDCL2DlDCL3 and DlDCL4 were upregulated significantly in high concentration ETH treatment, while DlDCL1 expression was significantly up-regulated in low concentration (0.1%) treatment with DlDCL2DlDCL3 and DlDCL3 with high sucrose concentration. The relative expression of DlDCL3 decreased with the increase of temperature at 34 鈩，

本文编号：1800588