水稻卷叶突变体rlm1的基因克隆

发布时间：2018-04-26 09:32

本文选题：水稻(Oryza + sativa　；参考：《中国农业科技导报》2016年02期

【摘要】：叶片是植物光合作用的主要场所,优良的叶片形态有利于塑造理想的株型,提高光合效率。为了研究叶片形态建成的分子机制,自水稻T-DNA插入突变体库中筛选获得1个叶片半卷曲的卷叶突变体(roll leaf mutant,命名为rlm1),突变体rlm1主要特征为成熟叶片沿中脉向内卷,叶片最终卷成直立半圆筒状,叶片卷曲度达0.64,叶片直立参数达95,且光合效率显著优于野生型。通过图位克隆技术,确定突变体rlm1突变位点位于LOC_Os03g06654基因的第1个内含子,LOC_Os03g06654基因编码黄素单氧化酶(flavin-containing monooxygenase),RT-PCR表达分析表明LOC_Os03g06654基因因T-DNA插入而导致完全失活。该基因与已报道的水稻卷叶基因Os COW1(Constitutively Wilted 1)为等位基因,而且突变体rlm1所表现的农艺性状均佳,可期待利用该突变体进行高光合的育种实践。
[Abstract]:Leaf is the main place of plant photosynthesis. Good leaf morphology is beneficial to shape ideal plant type and improve photosynthetic efficiency. In order to study the molecular mechanism of leaf morphogenesis, a roll leaf mutant, named rlm1a, was obtained from rice T-DNA insertion mutant library. The main feature of the mutant rlm1 is that mature leaves roll inward along the midvein. The leaf was finally rolled into an upright semi-cylindrical shape with a leaf curvature of 0.64 and a leaf vertical parameter of 95. The photosynthetic efficiency was significantly higher than that of the wild type. By using map-based cloning technique, the mutation site of the mutant rlm1 was confirmed to be located in the first intron of the LOC_Os03g06654 gene. RT-PCR analysis showed that the LOC_Os03g06654 gene was completely inactivated due to the insertion of T-DNA, which was encoded by flavin-containing monooxygenase of Flavin monooxygenase in the gene Os03g06654. This gene is allelic to the reported rice leaf winding gene Os COW1(Constitutively Wilted 1, and the mutant rlm1 shows good agronomic characters. It can be expected that the mutant can be used for high photozygosity breeding.
【作者单位】：首都师范大学生命科学学院;中国农业科学院生物技术研究所;
【基金】：中国-巴西作物生物技术与种子资源联合研究项目(2014DFA31550)资助
【分类号】：S511

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